The glucose-sensitive multilayer films composed of concanavalin A (Con A)-dextran were studied for developing optical glucose sensors. The multilayer films were prepared on the surface of a quartz slide, which was treated in diclorodimethylsilane overnoght at room temperature. It was reported that the Con A-glycogen multilayer films were sensitive to sugars and can be fully disintegrated in the presence of D-glucose, D-mannose, and derivatives. The sugar-induced disintegration of Con A-glycogen films was evaluated by measuring absorption spectra of the films. The stability of the Con A-glycogen multilayer films, in the sugar-free buffer solutions at pH 6, 7, and 8, was also studied.