Complete 1H and 13C resonance assignments were carried out for a new type of carboxyl-linked glucosides of chenodeoxycholic (3α,7α-dihydroxy-5β-cholan-24-oic) and hyodeoxycholic (3α,6α-dihydroxy-5β-cholan24-oic) acids by using several homonuclear (1H-1H) and heteronuclear (1H- 13C) 2D NMR techniques. Differences in the 1H and 13C resonances between the α- and β-anomers of the ester glucosides of bile acids were clarified for the first time. A comparison of the 1H and 13C signal shifts induced by β -D-glucosidation at the 24-carboxyl and 3α-hydroxyl groups in the parent 5β-cholanoic acid was also made.
- Bile acid esters of glucose
- C NMR
- Carboxyl-linked glucosides of bile acids
- Glycosidic conjugates of bile acids
- H NMR
- H-C shift-correlated 2D NMR
- H-H shift-correlated 2D NMR