1HMRI detection of gene expression in living cells by using protein tag and biotinylation probe

Akimasa Yoshimura; Shin Mizukami; Yuki Mori; Yoshichika Yoshioka; Kazuya Kikuchi

doi:10.1246/cl.130942

¹HMRI detection of gene expression in living cells by using protein tag and biotinylation probe

Akimasa Yoshimura, Shin Mizukami, Yuki Mori, Yoshichika Yoshioka, Kazuya Kikuchi

IMRAM - Division of Organic- and Biomaterials Research

Osaka University

Research output: Contribution to journal › Article › peer-review

Abstract

A new detection method for gene expression by magnetic resonance imaging (MRI) was developed using a proteinlabeling system based on a noncatalytic mutant β-lactamase (BL-tag system). By combining the BL-tag system with a specific biotinylation probe and a streptavidin-conjugated superparamagnetic iron oxide, target gene expression in living cells was successful detected by ¹HMRI.

Original language	English
Pages (from-to)	219-221
Number of pages	3
Journal	Chemistry Letters
Volume	43
Issue number	2
DOIs	https://doi.org/10.1246/cl.130942
Publication status	Published - 2014

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10.1246/cl.130942

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title = "1HMRI detection of gene expression in living cells by using protein tag and biotinylation probe",

abstract = "A new detection method for gene expression by magnetic resonance imaging (MRI) was developed using a proteinlabeling system based on a noncatalytic mutant β-lactamase (BL-tag system). By combining the BL-tag system with a specific biotinylation probe and a streptavidin-conjugated superparamagnetic iron oxide, target gene expression in living cells was successful detected by 1HMRI.",

author = "Akimasa Yoshimura and Shin Mizukami and Yuki Mori and Yoshichika Yoshioka and Kazuya Kikuchi",

year = "2014",

doi = "10.1246/cl.130942",

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journal = "Chemistry Letters",

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T1 - 1HMRI detection of gene expression in living cells by using protein tag and biotinylation probe

AU - Yoshimura, Akimasa

AU - Mizukami, Shin

AU - Mori, Yuki

AU - Yoshioka, Yoshichika

AU - Kikuchi, Kazuya

PY - 2014

Y1 - 2014

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AB - A new detection method for gene expression by magnetic resonance imaging (MRI) was developed using a proteinlabeling system based on a noncatalytic mutant β-lactamase (BL-tag system). By combining the BL-tag system with a specific biotinylation probe and a streptavidin-conjugated superparamagnetic iron oxide, target gene expression in living cells was successful detected by 1HMRI.

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¹HMRI detection of gene expression in living cells by using protein tag and biotinylation probe

Abstract

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