Super-wide-field two-photon imaging with a micro-optical device moving in post-objective space

Shin Ichiro Terada, Kenta Kobayashi, Masamichi Ohkura, Junichi Nakai, Masanori Matsuzaki

Research output: Contribution to journalArticlepeer-review

35 Citations (Scopus)


Wide-field imaging of neural activity at a cellular resolution is a current challenge in neuroscience. To address this issue, wide-field two-photon microscopy has been developed; however, the field size is limited by the objective size. Here, we develop a micro-opto-mechanical device that rotates within the post-objective space between the objective and brain tissue. Two-photon microscopy with this device enables sub-second sequential calcium imaging of left and right mouse sensory forelimb areas 6 mm apart. When imaging the rostral and caudal motor forelimb areas (RFA and CFA) 2 mm apart, we found high pairwise correlations in spontaneous activity between RFA and CFA neurons and between an RFA neuron and its putative axons in CFA. While mice performed a sound-triggered forelimb-movement task, the population activity between RFA and CFA covaried across trials, although the field-averaged activity was similar across trials. The micro-opto-mechanical device in the post-objective space provides a novel and flexible design to clarify the correlation structure between distant brain areas at subcellular and population levels.

Original languageEnglish
Article number3550
JournalNature Communications
Issue number1
Publication statusPublished - 2018 Dec 1


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