TY - JOUR
T1 - Superoxide dismutase in normal cycling human ovaries
T2 - Immunohistochemical localization and characterization
AU - Suzuki, Takashi
AU - Sugino, Norihiro
AU - Fukaya, Takao
AU - Sugiyama, Shinichiro
AU - Uda, Taizo
AU - Takaya, Risa
AU - Yajima, Akira
AU - Sasano, Hironobu
PY - 1999/10
Y1 - 1999/10
N2 - Objective: To investigate the expression of manganese (Mn) and copper- zinc (Cu,Zn) superoxide dismutase (SOD) in normal cycling human ovaries throughout the menstrual cycle. Design: Descriptive, controlled study. Setting: Tohoku University School of Medicine. Patient(s): Twenty-four normal cycling human ovaries were obtained from patients who underwent oophorectomy and hysterectomy for squamous cell carcinoma of the uterine cervix. Intervention(s): Immunohistochemistry for Mn-SOD and Cu,Zn-SOD. Main Outcome Measure(s): Immunostaining. Result(s): In the follicular stage, Mn-SOD immunoreactivity was detected in granulosa and theca interna cells of steroid-producing follicles, that is, preantral, nondominant, dominant, and atretic follicles, whereas Cu,Zn-SOD was detected in theca interna cells of these follicles and in granulosa cells of dominant follicles. In the luteal stage, immunoreactivity for Mn-SOD and Cu,Zn-SOD was observed in both luteinized granulosa and theca cells of the functioning corpus luteum. In the early degenerating corpus luteum, both Mn-SOD and Cu,Zn-SOD were positive in steroid-producing luteinized theca cells. Mn-SOD immunoreactivity was also detected in nonsteroid-producing luteinized granulosa cells and macrophages. Conclusion(s): Our results suggest that the expression of Mn-SOD and Cu,Zn- SOD closely correlates with steroidogenesis in the human ovary. In addition, Mn-SOD may play an important role in the process of luteal regression.
AB - Objective: To investigate the expression of manganese (Mn) and copper- zinc (Cu,Zn) superoxide dismutase (SOD) in normal cycling human ovaries throughout the menstrual cycle. Design: Descriptive, controlled study. Setting: Tohoku University School of Medicine. Patient(s): Twenty-four normal cycling human ovaries were obtained from patients who underwent oophorectomy and hysterectomy for squamous cell carcinoma of the uterine cervix. Intervention(s): Immunohistochemistry for Mn-SOD and Cu,Zn-SOD. Main Outcome Measure(s): Immunostaining. Result(s): In the follicular stage, Mn-SOD immunoreactivity was detected in granulosa and theca interna cells of steroid-producing follicles, that is, preantral, nondominant, dominant, and atretic follicles, whereas Cu,Zn-SOD was detected in theca interna cells of these follicles and in granulosa cells of dominant follicles. In the luteal stage, immunoreactivity for Mn-SOD and Cu,Zn-SOD was observed in both luteinized granulosa and theca cells of the functioning corpus luteum. In the early degenerating corpus luteum, both Mn-SOD and Cu,Zn-SOD were positive in steroid-producing luteinized theca cells. Mn-SOD immunoreactivity was also detected in nonsteroid-producing luteinized granulosa cells and macrophages. Conclusion(s): Our results suggest that the expression of Mn-SOD and Cu,Zn- SOD closely correlates with steroidogenesis in the human ovary. In addition, Mn-SOD may play an important role in the process of luteal regression.
KW - Ad4-binding protein (Ad4BP)
KW - Human ovary
KW - Immunohistochemistry
KW - Steroidogenesis
KW - Superoxide dismutase (SOD)
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U2 - 10.1016/S0015-0282(99)00332-5
DO - 10.1016/S0015-0282(99)00332-5
M3 - Article
C2 - 10521117
AN - SCOPUS:0033214057
SN - 0015-0282
VL - 72
SP - 720
EP - 726
JO - Fertility and Sterility
JF - Fertility and Sterility
IS - 4
ER -
