@inproceedings{b4889d9fdd364d419866355fe34f7aaf,
title = "Surface-enhanced Raman scattering (SERS) imaging of alkyne-tagged small molecule drug in live cells with endocytosed gold nanoparticles",
abstract = "We propose the combination of alkyne-tag and surface-enhanced Raman scattering (SERS) spectroscopy to perform highly-sensitive and selective drug imaging in live cells. Gold nanoparticles are introduced in lysosomes through endocytosis as SERS agents, and the alkyne-tagged drugs are subsequently administered in cells. Raman microscopic observation reveals the arrival of drug in lysosome through enhanced Raman signal of alkyne. Since the peak of alkyne appears in Raman-silent region of biomolecules, selective detection of drugs is possible without background signal of endogenous molecules. From endocytosed gold nanoparticles in living HeLa cells, we observed distinct Raman signal from alkyne-tagged inhibitor of lysosomal enzyme.",
keywords = "alkyne-tag, gold nanoparticle, live cell, Raman imaging, small molecule, Surface-enhanced Raman scattering",
author = "Jun Ando and Takumasa Sekiya and Den Ka and Hiroyuki Yamakoshi and Kosuke Dodo and Mikiko Sodeoka and Satoshi Kawata and Katsumasa Fujita",
note = "Publisher Copyright: {\textcopyright} 2017 SPIE.; Visualizing and Quantifying Drug Distribution in Tissue ; Conference date: 28-01-2017",
year = "2017",
doi = "10.1117/12.2256126",
language = "English",
series = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",
publisher = "SPIE",
editor = "Evans, {Conor L.} and Chan, {Kin Foong}",
booktitle = "Visualizing and Quantifying Drug Distribution in Tissue",
}