Surface structures and osteoblast response of hydrothermally produced CaTiO 3 thin film on Ti-13Nb-13Zr alloy

Jin Woo Park, Yusuke Tustusmi, Chong Soo Lee, Chan Hee Park, Youn Jeong Kim, Je Hee Jang, Dongwoo Khang, Yeon Min Im, Hisashi Doi, Naoyuki Nomura, Takao Hanawa

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23 Citations (Scopus)


This study investigated the surface characteristics and in vitro biocompatibility of a titanium (Ti) oxide layer incorporating calcium ions (Ca) obtained by hydrothermal treatment with or without post heat-treatment in the Ti-13Nb-13Zr alloy. The surface characteristics were evaluated by scanning electron microscopy, thin-film X-ray diffractometry, X-ray photoelectron spectroscopy, atomic force microscopy and contact angle measurements. In vitro biocompatibility of the Ca-containing surfaces was assessed in comparison with untreated surfaces using a pre-osteoblast cell line. Hydrothermal treatment produced a crystalline CaTiO 3 layer. Post heat-treatment at 400 °C for 2 h in air significantly decreased water contact angles in the CaTiO 3 layer (p < 0.001). The Ca-incorporated alloy surfaces displayed markedly increased cell viability and ALP activity compared with untreated surfaces (p < 0.001), and also an upregulated expression of various integrin genes (α1, α2, α5, αv, β1 and β3) at an early incubation time-point. Post heat-treatment further increased attachment and ALP activity in cells grown on Ca-incorporated Ti-13Nb-13Zr alloy surfaces. The results indicate that the Ca-incorporated oxide layer produced by hydrothermal treatment and a simple post heat-treatment may be effective in improving bone healing in Ti-13Nb-13Zr alloy implants by enhancing the viability and differentiation of osteoblastic cells.

Original languageEnglish
Pages (from-to)7856-7863
Number of pages8
JournalApplied Surface Science
Issue number17
Publication statusPublished - 2011 Jun 15


  • Calcium ion
  • Hydrothermal treatment
  • Osteoblast
  • Surface hydrophilicity
  • Ti-13Nb-13Zr alloy


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