Surface structures and osteoblast response of hydrothermally produced CaTiO                         3                          thin film on Ti-13Nb-13Zr alloy

Jin Woo Park; Yusuke Tustusmi; Chong Soo Lee; Chan Hee Park; Youn Jeong Kim; Je Hee Jang; Dongwoo Khang; Yeon Min Im; Hisashi Doi; Naoyuki Nomura; Takao Hanawa

doi:10.1016/j.apsusc.2011.04.054

Surface structures and osteoblast response of hydrothermally produced CaTiO ₃ thin film on Ti-13Nb-13Zr alloy

Jin Woo Park, Yusuke Tustusmi, Chong Soo Lee, Chan Hee Park, Youn Jeong Kim, Je Hee Jang, Dongwoo Khang, Yeon Min Im, Hisashi Doi, Naoyuki Nomura, Takao Hanawa

ENG - Materials Processing

Research output: Contribution to journal › Article › peer-review

23 Citations (Scopus)

Abstract

This study investigated the surface characteristics and in vitro biocompatibility of a titanium (Ti) oxide layer incorporating calcium ions (Ca) obtained by hydrothermal treatment with or without post heat-treatment in the Ti-13Nb-13Zr alloy. The surface characteristics were evaluated by scanning electron microscopy, thin-film X-ray diffractometry, X-ray photoelectron spectroscopy, atomic force microscopy and contact angle measurements. In vitro biocompatibility of the Ca-containing surfaces was assessed in comparison with untreated surfaces using a pre-osteoblast cell line. Hydrothermal treatment produced a crystalline CaTiO ₃ layer. Post heat-treatment at 400 °C for 2 h in air significantly decreased water contact angles in the CaTiO ₃ layer (p < 0.001). The Ca-incorporated alloy surfaces displayed markedly increased cell viability and ALP activity compared with untreated surfaces (p < 0.001), and also an upregulated expression of various integrin genes (α1, α2, α5, αv, β1 and β3) at an early incubation time-point. Post heat-treatment further increased attachment and ALP activity in cells grown on Ca-incorporated Ti-13Nb-13Zr alloy surfaces. The results indicate that the Ca-incorporated oxide layer produced by hydrothermal treatment and a simple post heat-treatment may be effective in improving bone healing in Ti-13Nb-13Zr alloy implants by enhancing the viability and differentiation of osteoblastic cells.

Original language	English
Pages (from-to)	7856-7863
Number of pages	8
Journal	Applied Surface Science
Volume	257
Issue number	17
DOIs	https://doi.org/10.1016/j.apsusc.2011.04.054
Publication status	Published - 2011 Jun 15

Keywords

Calcium ion
Hydrothermal treatment
Osteoblast
Surface hydrophilicity
Ti-13Nb-13Zr alloy

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10.1016/j.apsusc.2011.04.054

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Park, J. W., Tustusmi, Y., Lee, C. S., Park, C. H., Kim, Y. J., Jang, J. H., Khang, D., Im, Y. M., Doi, H., Nomura, N., & Hanawa, T. (2011). Surface structures and osteoblast response of hydrothermally produced CaTiO ₃ thin film on Ti-13Nb-13Zr alloy Applied Surface Science, 257(17), 7856-7863. https://doi.org/10.1016/j.apsusc.2011.04.054

@article{ef2cfe0d538e4c9dad2622099e4b0d02,

title = " Surface structures and osteoblast response of hydrothermally produced CaTiO 3 thin film on Ti-13Nb-13Zr alloy ",

abstract = " This study investigated the surface characteristics and in vitro biocompatibility of a titanium (Ti) oxide layer incorporating calcium ions (Ca) obtained by hydrothermal treatment with or without post heat-treatment in the Ti-13Nb-13Zr alloy. The surface characteristics were evaluated by scanning electron microscopy, thin-film X-ray diffractometry, X-ray photoelectron spectroscopy, atomic force microscopy and contact angle measurements. In vitro biocompatibility of the Ca-containing surfaces was assessed in comparison with untreated surfaces using a pre-osteoblast cell line. Hydrothermal treatment produced a crystalline CaTiO 3 layer. Post heat-treatment at 400 °C for 2 h in air significantly decreased water contact angles in the CaTiO 3 layer (p < 0.001). The Ca-incorporated alloy surfaces displayed markedly increased cell viability and ALP activity compared with untreated surfaces (p < 0.001), and also an upregulated expression of various integrin genes (α1, α2, α5, αv, β1 and β3) at an early incubation time-point. Post heat-treatment further increased attachment and ALP activity in cells grown on Ca-incorporated Ti-13Nb-13Zr alloy surfaces. The results indicate that the Ca-incorporated oxide layer produced by hydrothermal treatment and a simple post heat-treatment may be effective in improving bone healing in Ti-13Nb-13Zr alloy implants by enhancing the viability and differentiation of osteoblastic cells.",

keywords = "Calcium ion, Hydrothermal treatment, Osteoblast, Surface hydrophilicity, Ti-13Nb-13Zr alloy",

author = "Park, {Jin Woo} and Yusuke Tustusmi and Lee, {Chong Soo} and Park, {Chan Hee} and Kim, {Youn Jeong} and Jang, {Je Hee} and Dongwoo Khang and Im, {Yeon Min} and Hisashi Doi and Naoyuki Nomura and Takao Hanawa",

note = "Funding Information: This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (No. R13-2010-0029494 ), Republic of Korea.",

year = "2011",

month = jun,

day = "15",

doi = "10.1016/j.apsusc.2011.04.054",

language = "English",

volume = "257",

pages = "7856--7863",

journal = "Applied Surface Science",

issn = "0169-4332",

publisher = "Elsevier",

number = "17",

}

Park, JW, Tustusmi, Y, Lee, CS, Park, CH, Kim, YJ, Jang, JH, Khang, D, Im, YM, Doi, H, Nomura, N & Hanawa, T 2011, ' Surface structures and osteoblast response of hydrothermally produced CaTiO ₃ thin film on Ti-13Nb-13Zr alloy ', Applied Surface Science, vol. 257, no. 17, pp. 7856-7863. https://doi.org/10.1016/j.apsusc.2011.04.054

Surface structures and osteoblast response of hydrothermally produced CaTiO ₃ thin film on Ti-13Nb-13Zr alloy . / Park, Jin Woo; Tustusmi, Yusuke; Lee, Chong Soo et al.
In: Applied Surface Science, Vol. 257, No. 17, 15.06.2011, p. 7856-7863.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Surface structures and osteoblast response of hydrothermally produced CaTiO 3 thin film on Ti-13Nb-13Zr alloy

AU - Park, Jin Woo

AU - Tustusmi, Yusuke

AU - Lee, Chong Soo

AU - Park, Chan Hee

AU - Kim, Youn Jeong

AU - Jang, Je Hee

AU - Khang, Dongwoo

AU - Im, Yeon Min

AU - Doi, Hisashi

AU - Nomura, Naoyuki

AU - Hanawa, Takao

N1 - Funding Information: This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (No. R13-2010-0029494 ), Republic of Korea.

PY - 2011/6/15

Y1 - 2011/6/15

N2 - This study investigated the surface characteristics and in vitro biocompatibility of a titanium (Ti) oxide layer incorporating calcium ions (Ca) obtained by hydrothermal treatment with or without post heat-treatment in the Ti-13Nb-13Zr alloy. The surface characteristics were evaluated by scanning electron microscopy, thin-film X-ray diffractometry, X-ray photoelectron spectroscopy, atomic force microscopy and contact angle measurements. In vitro biocompatibility of the Ca-containing surfaces was assessed in comparison with untreated surfaces using a pre-osteoblast cell line. Hydrothermal treatment produced a crystalline CaTiO 3 layer. Post heat-treatment at 400 °C for 2 h in air significantly decreased water contact angles in the CaTiO 3 layer (p < 0.001). The Ca-incorporated alloy surfaces displayed markedly increased cell viability and ALP activity compared with untreated surfaces (p < 0.001), and also an upregulated expression of various integrin genes (α1, α2, α5, αv, β1 and β3) at an early incubation time-point. Post heat-treatment further increased attachment and ALP activity in cells grown on Ca-incorporated Ti-13Nb-13Zr alloy surfaces. The results indicate that the Ca-incorporated oxide layer produced by hydrothermal treatment and a simple post heat-treatment may be effective in improving bone healing in Ti-13Nb-13Zr alloy implants by enhancing the viability and differentiation of osteoblastic cells.

AB - This study investigated the surface characteristics and in vitro biocompatibility of a titanium (Ti) oxide layer incorporating calcium ions (Ca) obtained by hydrothermal treatment with or without post heat-treatment in the Ti-13Nb-13Zr alloy. The surface characteristics were evaluated by scanning electron microscopy, thin-film X-ray diffractometry, X-ray photoelectron spectroscopy, atomic force microscopy and contact angle measurements. In vitro biocompatibility of the Ca-containing surfaces was assessed in comparison with untreated surfaces using a pre-osteoblast cell line. Hydrothermal treatment produced a crystalline CaTiO 3 layer. Post heat-treatment at 400 °C for 2 h in air significantly decreased water contact angles in the CaTiO 3 layer (p < 0.001). The Ca-incorporated alloy surfaces displayed markedly increased cell viability and ALP activity compared with untreated surfaces (p < 0.001), and also an upregulated expression of various integrin genes (α1, α2, α5, αv, β1 and β3) at an early incubation time-point. Post heat-treatment further increased attachment and ALP activity in cells grown on Ca-incorporated Ti-13Nb-13Zr alloy surfaces. The results indicate that the Ca-incorporated oxide layer produced by hydrothermal treatment and a simple post heat-treatment may be effective in improving bone healing in Ti-13Nb-13Zr alloy implants by enhancing the viability and differentiation of osteoblastic cells.

KW - Calcium ion

KW - Hydrothermal treatment

KW - Osteoblast

KW - Surface hydrophilicity

KW - Ti-13Nb-13Zr alloy

UR - http://www.scopus.com/inward/record.url?scp=79957449593&partnerID=8YFLogxK

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U2 - 10.1016/j.apsusc.2011.04.054

DO - 10.1016/j.apsusc.2011.04.054

M3 - Article

AN - SCOPUS:79957449593

SN - 0169-4332

VL - 257

SP - 7856

EP - 7863

JO - Applied Surface Science

JF - Applied Surface Science

IS - 17

ER -

Surface structures and osteoblast response of hydrothermally produced CaTiO ₃ thin film on Ti-13Nb-13Zr alloy

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