TY - JOUR
T1 - Surfactant-Free Decellularization of Porcine Aortic Tissue by Subcritical Dimethyl Ether
AU - Kanda, Hideki
AU - Ando, Daigo
AU - Hoshino, Rintaro
AU - Yamamoto, Tetsuya
AU - Wahyudiono,
AU - Suzuki, Shogo
AU - Shinohara, Satoshi
AU - Goto, Motonobu
N1 - Funding Information:
This research was supported by Japan Society for the Promotion of Science KAKENHI (grant numbers 17K06922 and JP20K05192 to H.K.), Science and Technology Research Partnership for Sustainable Development Program of Japan Science and Technology Agency (JST) and Japan International Cooperation Agency (JICA) (grant number JPMJSA1505 to H.K.), Tatematsu Foundation, and Ito Foundation. The authors are grateful to Prof. Akio Kishida (Tokyo Medical and Dental University) for their kind help and constructive suggestions.
Funding Information:
This research was supported by Japan Society for the Promotion of Science KAKENHI (grant numbers 17K06922 and JP20K05192 to H.K.), Science and Technology Research Partnership for Sustainable Development Program of Japan Science and Technology Agency (JST), and Japan International Cooperation Agency (JICA) (grant number JPMJSA1505 to H.K.), Tatematsu Foundation, and Ito Foundation. The authors are grateful to Prof. Akio Kishida (Tokyo Medical and Dental University) for their kind help and constructive suggestions.
Publisher Copyright:
©
PY - 2021/5/25
Y1 - 2021/5/25
N2 - Porcine aortic tissue was decellularized by subcritical dimethyl ether (DME) used as an alternative to the surfactant sodium dodecyl sulfate. The process included three steps. For the first step, lipids were extracted from the porcine aorta using subcritical DME at 23 °C with a DME pressure of 0.56 MPa. Next, DME was evaporated from the aorta under atmospheric pressure and temperature. The second step involved DNA fragmentation by DNase, which was primarily identical to the common method. For the third step, similar to the common method, DNA fragments were removed by washing with water and ethanol. After 3 days of DNase treatment, the amount of DNA remaining in the porcine aorta was 40 ng/dry-mg, which was lower than the standard value of 50 ng/mg-dry. Hematoxylin and eosin staining showed that most cell nuclei were removed from the aorta. These results demonstrate that subcritical DME eliminates the need to utilize surfactants.
AB - Porcine aortic tissue was decellularized by subcritical dimethyl ether (DME) used as an alternative to the surfactant sodium dodecyl sulfate. The process included three steps. For the first step, lipids were extracted from the porcine aorta using subcritical DME at 23 °C with a DME pressure of 0.56 MPa. Next, DME was evaporated from the aorta under atmospheric pressure and temperature. The second step involved DNA fragmentation by DNase, which was primarily identical to the common method. For the third step, similar to the common method, DNA fragments were removed by washing with water and ethanol. After 3 days of DNase treatment, the amount of DNA remaining in the porcine aorta was 40 ng/dry-mg, which was lower than the standard value of 50 ng/mg-dry. Hematoxylin and eosin staining showed that most cell nuclei were removed from the aorta. These results demonstrate that subcritical DME eliminates the need to utilize surfactants.
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U2 - 10.1021/acsomega.1c01549
DO - 10.1021/acsomega.1c01549
M3 - Article
AN - SCOPUS:85106503089
SN - 2470-1343
VL - 6
SP - 13417
EP - 13425
JO - ACS Omega
JF - ACS Omega
IS - 20
ER -