Suspension culture of Nicotiana tabacum cells in a rotary‐drum bioreactor

Naomi Shibasaki, Kenji Hirose, Toshikuni Yonemoto, Teiriki Tadaki

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25 Citations (Scopus)


Suspension cultures of Nicotiana tabacum (tobacco) were studied using a rotary‐drum bioreactor and the effects of various operating factors on culture growth were investigated. Conditions of oxygen concentration, aeration and drum rotation rates required for optimum culture growth rate were determined. The results were compared with those obtained in an air‐lift bioreactor with a draft tube. The culture growth rates were higher in the rotary‐drum bioreactor than in the air‐lift bioreactor under the same conditions of aeration. The rotary‐drum bioreactor was superior to the air‐lift bioreactor in respect of oxygen mass transfer and the level of shear damage to N. tabacum cells.

Original languageEnglish
Pages (from-to)359-363
Number of pages5
JournalJournal of Chemical Technology and Biotechnology
Issue number4
Publication statusPublished - 1992


  • Nicotiana tabacum
  • air‐lift bioreactor
  • roller bottle
  • rotary‐drum bioreactor
  • suspension culture


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