Synergistic effect of proanthocyanidin on the bactericidal action of the photolysis of H2O2

Hiroyo Ikai, Keisuke Nakamura, Taro Kanno, Midori Shirato, Luiz Meirelles, Keiichi Sasaki, Yoshimi Niwano

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)


The in vitro antibacterial activity of the hydroxyl radical generation system by the photolysis of H2O2 in combination with proanthocyanidin, which refers to a group of polyphenolic compounds, was examined. Bactericidal activity of photo-irradiated H2O2 at 405 nm against Streptococcus mutans, a major pathogen of dental caries, was augmented in the presence of proanthocyanidin, whose bactericidal effect by itself was very poor, in a concentration-dependent manner. This combination was also proven effective against Porphyromonas gingivalis, a major pathogen of periodontitis. It is speculated that H2O2, generated from photo-irradiated proanthocyanidin around the bacterial cells, is photolyzed to the hydroxyl radical, which would in turn affect the membrane structure and function of the bacterial cells, resulting in augmented sensitivity of bacterial cells to the disinfection system utilizing the photolysis of H 2O2. The present study suggests that the combination of H2O2 and proanthocyanidin works synergistically to kill bacteria when photo-irradiated.

Original languageEnglish
Pages (from-to)137-141
Number of pages5
JournalBiocontrol Science
Issue number3
Publication statusPublished - 2013


  • Bactericidal activity
  • Photolysis of HO
  • Porphyromonas gingivalis
  • Proanthocayanidin
  • Streptococcus mutans


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