Synthesis and characterization of sapecin and sapecin B

Jae Il Kim, Hideo Iwai, Shoichiro Kurata, Masami Takahashi, Katuyoshi Masuda, Ichio Shimada, Shunji Natori, Yoji Arata, Kazuki Sato

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)


Two insect defencins, sapecin and sapecin B, were chemically synthesized to confirm their structure and antibacterial activity and also to examine the possibility that these peptides bind to the same site on the large conductance calcium-activated potassium channel as charybdotoxin. Both synthetic peptides showed the same antibacterial activity as native sapecins, indicating that the synthetic peptides folded correctly in the chemical synthesis. Synthetic sapecins did not show an inhibitory effect on [125I]charybdotoxin binding to rat brain synaptic membranes, suggesting that sapecin B recognizes a different binding site from that of charybdotoxin despite the similar structural motif.

Original languageEnglish
Pages (from-to)189-192
Number of pages4
JournalFEBS Letters
Issue number2
Publication statusPublished - 1994 Apr 4


  • Charybdotoxin
  • Potassium channel
  • Sapecin
  • Sapecin B


Dive into the research topics of 'Synthesis and characterization of sapecin and sapecin B'. Together they form a unique fingerprint.

Cite this