A new method for the synthesis of ODN-luciferase conjugate was investigated as a signal-amplifying sensor of the target nucleic acids. The conjugation of the luciferase was successfully achieved between the cysteine residue and the 2-amino-6-vinylpurine nucleoside of the ODN probe without significant inactivation of luciferase. The ODN-luciferase conjugate modified with PEG retained the luciferase activity and selectivity during the hybridization with the target ODN.
|Number of pages||6|
|Journal||Archiv der Pharmazie|
|Publication status||Published - 2008 Sept|
- Nucleic acid
ASJC Scopus subject areas
- Pharmaceutical Science
- Drug Discovery