Synthesis of a bridging ligand with a non-denatured protein pendant: Toward protein encapsulation in a coordination cage

Daishi Fujita; Kosuke Suzuki; Sota Sato; Maho Yagi-Utsumi; Eiji Kurimoto; Yoshiki Yamaguchi; Koichi Kato; Makoto Fujita

doi:10.1246/cl.2012.313

Synthesis of a bridging ligand with a non-denatured protein pendant: Toward protein encapsulation in a coordination cage

Daishi Fujita, Kosuke Suzuki, Sota Sato, Maho Yagi-Utsumi, Eiji Kurimoto, Yoshiki Yamaguchi, Koichi Kato, Makoto Fujita

Research output: Contribution to journal › Article › peer-review

13 Citations (Scopus)

Abstract

Toward protein encapsulation by a synthetic host, we synthesized an ubiquitin-dangled ligand, a potential precursor of a nanoscale coordination cage. The key step is the addition of a C-terminal Cys76 SH group, which was introduced by Gly76Cys mutation, to a maleimide acceptor on the ligand. The C-terminal mutation and the SH addition to the ligand did not damage the ubiquitin moiety at all, neither structurally nor conformationally.

Original language	English
Pages (from-to)	313-315
Number of pages	3
Journal	Chemistry Letters
Volume	41
Issue number	3
DOIs	https://doi.org/10.1246/cl.2012.313
Publication status	Published - 2012

ASJC Scopus subject areas

Chemistry(all)

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10.1246/cl.2012.313

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abstract = "Toward protein encapsulation by a synthetic host, we synthesized an ubiquitin-dangled ligand, a potential precursor of a nanoscale coordination cage. The key step is the addition of a C-terminal Cys76 SH group, which was introduced by Gly76Cys mutation, to a maleimide acceptor on the ligand. The C-terminal mutation and the SH addition to the ligand did not damage the ubiquitin moiety at all, neither structurally nor conformationally.",

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T2 - Toward protein encapsulation in a coordination cage

AU - Fujita, Daishi

AU - Suzuki, Kosuke

AU - Sato, Sota

AU - Yagi-Utsumi, Maho

AU - Kurimoto, Eiji

AU - Yamaguchi, Yoshiki

AU - Kato, Koichi

AU - Fujita, Makoto

PY - 2012

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AB - Toward protein encapsulation by a synthetic host, we synthesized an ubiquitin-dangled ligand, a potential precursor of a nanoscale coordination cage. The key step is the addition of a C-terminal Cys76 SH group, which was introduced by Gly76Cys mutation, to a maleimide acceptor on the ligand. The C-terminal mutation and the SH addition to the ligand did not damage the ubiquitin moiety at all, neither structurally nor conformationally.

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JO - Chemistry Letters

JF - Chemistry Letters

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Synthesis of a bridging ligand with a non-denatured protein pendant: Toward protein encapsulation in a coordination cage

Abstract

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