@article{d1cc500ddaa94325b3509988b5d2a489,
title = "Synthesis of crosslinked 2′-OMe RNA duplexes and their application for effective inhibition of miRNA function",
abstract = "The interstrand crosslinking of nucleic acids is one of the strategies to create the stable complex between an oligonucleotide and RNA by covalent bond formation. We previously reported that fully 2′-O-methylated (2′-OMe) RNAs having the 2-amino-6-vinylpurine (AVP) exhibited an efficient crosslinking to uracil in the target RNA. In this study, we established a chemical method to efficiently synthesize the crosslinked 2′-OMe RNA duplexes using AVP and prepared the anti-miRNA oligonucleotides (AMOs) containing the antisense targeting miR-21 and crosslinked duplex at the terminal sequences. These AMOs showed a markedly higher anti miRNA activity than that of the commercially-available miR-21 inhibitor which has locked nucleic acid (LNA) residues.",
keywords = "Anti-miRNA, Crosslinked duplex, Oligonucleotides, RISC complex",
author = "Abdelhady, {Ahmed Mostafa} and Yu Hirano and Kazumitsu Onizuka and Hidenori Okamura and Yasuo Komatsu and Fumi Nagatsugi",
note = "Funding Information: This work was supported by a Grant-in-Aid for Scientific Research on Innovative Areas (“Middle Molecular Strategy” JP15H05838) and a Grant-in-Aid for Scientific Research (B) [25288073], a Grant-in-Aid for Scientific Research (B) (No. JP19H02845 to K.O.), “Frontier Research on Chemical Communications” (No. JP20H04762 to K.O.) from the Japan Society for the Promotion of Science (JSPS). The first author (A.M.A) is funded by a full scholarship from the Ministry of Higher Education of the Arab Republic of Egypt and he would like to express his deepest gratitude for the financial support. Publisher Copyright: {\textcopyright} 2021 Elsevier Ltd",
year = "2021",
month = sep,
day = "15",
doi = "10.1016/j.bmcl.2021.128257",
language = "English",
volume = "48",
journal = "Bioorganic and Medicinal Chemistry Letters",
issn = "0960-894X",
publisher = "Elsevier Ltd.",
}