Synthesis of fluorescent-labeled aeruginosin derivatives for high-throughput fluorescence correlation spectroscopy assays

Yoichiro Hoshina; Yoshifumi Yamada; Hiroshi Tanaka; Takayuki Doi; Takashi Takahashi

doi:10.1016/j.bmcl.2007.02.045

Synthesis of fluorescent-labeled aeruginosin derivatives for high-throughput fluorescence correlation spectroscopy assays

Yoichiro Hoshina, Yoshifumi Yamada, Hiroshi Tanaka, Takayuki Doi, Takashi Takahashi

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

The design and solid-phase synthesis of effective fluorescent-labeled aeruginosin derivatives and their application to the fluorescence correlation spectroscopy (FCS)-based competitive binding assay of an aeruginosin library are described. The phenolic hydroxyl group on the (R)-3-(4-hydroxyphenyl)lactic acid (d-Hpla) residue was observed to be suitable for connecting Rhodamine green derivative with minimum loss of biological activity. In addition, the FCS-based binding assay of the library using fluorescent-labeled chemical probes was also achieved.

Original language	English
Pages (from-to)	2904-2907
Number of pages	4
Journal	Bioorganic and Medicinal Chemistry Letters
Volume	17
Issue number	10
DOIs	https://doi.org/10.1016/j.bmcl.2007.02.045
Publication status	Published - 2007 May 15
Externally published	Yes

Keywords

Chemical biology
Combinatorial chemistry
Fluorescence correlation spectroscopy
Solid-phase synthesis

ASJC Scopus subject areas

Biochemistry
Molecular Medicine
Molecular Biology
Pharmaceutical Science
Drug Discovery
Clinical Biochemistry
Organic Chemistry

Access to Document

10.1016/j.bmcl.2007.02.045

Cite this

@article{03671eefb7a146d59e693fe2dce07bd2,

title = "Synthesis of fluorescent-labeled aeruginosin derivatives for high-throughput fluorescence correlation spectroscopy assays",

abstract = "The design and solid-phase synthesis of effective fluorescent-labeled aeruginosin derivatives and their application to the fluorescence correlation spectroscopy (FCS)-based competitive binding assay of an aeruginosin library are described. The phenolic hydroxyl group on the (R)-3-(4-hydroxyphenyl)lactic acid (d-Hpla) residue was observed to be suitable for connecting Rhodamine green derivative with minimum loss of biological activity. In addition, the FCS-based binding assay of the library using fluorescent-labeled chemical probes was also achieved.",

keywords = "Chemical biology, Combinatorial chemistry, Fluorescence correlation spectroscopy, Solid-phase synthesis",

author = "Yoichiro Hoshina and Yoshifumi Yamada and Hiroshi Tanaka and Takayuki Doi and Takashi Takahashi",

note = "Funding Information: This work was supported in part by NEDO (the New Energy and Industrial Technology Development Organization) in Japan. ",

year = "2007",

month = may,

day = "15",

doi = "10.1016/j.bmcl.2007.02.045",

language = "English",

volume = "17",

pages = "2904--2907",

journal = "Bioorganic and Medicinal Chemistry Letters",

issn = "0960-894X",

publisher = "Elsevier Limited",

number = "10",

}

TY - JOUR

T1 - Synthesis of fluorescent-labeled aeruginosin derivatives for high-throughput fluorescence correlation spectroscopy assays

AU - Hoshina, Yoichiro

AU - Yamada, Yoshifumi

AU - Tanaka, Hiroshi

AU - Doi, Takayuki

AU - Takahashi, Takashi

N1 - Funding Information: This work was supported in part by NEDO (the New Energy and Industrial Technology Development Organization) in Japan.

PY - 2007/5/15

Y1 - 2007/5/15

N2 - The design and solid-phase synthesis of effective fluorescent-labeled aeruginosin derivatives and their application to the fluorescence correlation spectroscopy (FCS)-based competitive binding assay of an aeruginosin library are described. The phenolic hydroxyl group on the (R)-3-(4-hydroxyphenyl)lactic acid (d-Hpla) residue was observed to be suitable for connecting Rhodamine green derivative with minimum loss of biological activity. In addition, the FCS-based binding assay of the library using fluorescent-labeled chemical probes was also achieved.

AB - The design and solid-phase synthesis of effective fluorescent-labeled aeruginosin derivatives and their application to the fluorescence correlation spectroscopy (FCS)-based competitive binding assay of an aeruginosin library are described. The phenolic hydroxyl group on the (R)-3-(4-hydroxyphenyl)lactic acid (d-Hpla) residue was observed to be suitable for connecting Rhodamine green derivative with minimum loss of biological activity. In addition, the FCS-based binding assay of the library using fluorescent-labeled chemical probes was also achieved.

KW - Chemical biology

KW - Combinatorial chemistry

KW - Fluorescence correlation spectroscopy

KW - Solid-phase synthesis

UR - http://www.scopus.com/inward/record.url?scp=34247323956&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34247323956&partnerID=8YFLogxK

U2 - 10.1016/j.bmcl.2007.02.045

DO - 10.1016/j.bmcl.2007.02.045

M3 - Article

C2 - 17350836

AN - SCOPUS:34247323956

SN - 0960-894X

VL - 17

SP - 2904

EP - 2907

JO - Bioorganic and Medicinal Chemistry Letters

JF - Bioorganic and Medicinal Chemistry Letters

IS - 10

ER -

Synthesis of fluorescent-labeled aeruginosin derivatives for high-throughput fluorescence correlation spectroscopy assays

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this