Synthesis of peptide nucleic acids containing a crosslinking agent and evaluation of their reactivities

Takuya Akisawa; Yuki Ishizawa; Fumi Nagatsugi

doi:10.3390/molecules20034708

Synthesis of peptide nucleic acids containing a crosslinking agent and evaluation of their reactivities

Takuya Akisawa, Yuki Ishizawa, Fumi Nagatsugi

Division of Organic- and Biomaterials Research

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

Peptide nucleic acids (PNAs) are structural mimics of nucleic acids that form stable hybrids with DNA and RNA. In addition, PNAs can invade double-stranded DNA. Due to these characteristics, PNAs are widely used as biochemical tools, for example, in antisense/antigene therapy. Interstrand crosslink formation in nucleic acids is one of the strategies for preparing a stable duplex by covalent bond formation. In this study, we have synthesized PNAs incorporating 4-amino-6-oxo-2-vinylpyrimidine (AOVP) as a crosslinking agent and evaluated their reactivities for targeting DNA and RNA.

Original language	English
Pages (from-to)	4708-4719
Number of pages	12
Journal	Molecules
Volume	20
Issue number	3
DOIs	https://doi.org/10.3390/molecules20034708
Publication status	Published - 2015 Mar 1

Keywords

Antisense
Crosslink
Invasion
Peptide nucleic acid (PNA)

ASJC Scopus subject areas

Analytical Chemistry
Chemistry (miscellaneous)
Molecular Medicine
Pharmaceutical Science
Drug Discovery
Physical and Theoretical Chemistry
Organic Chemistry

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abstract = "Peptide nucleic acids (PNAs) are structural mimics of nucleic acids that form stable hybrids with DNA and RNA. In addition, PNAs can invade double-stranded DNA. Due to these characteristics, PNAs are widely used as biochemical tools, for example, in antisense/antigene therapy. Interstrand crosslink formation in nucleic acids is one of the strategies for preparing a stable duplex by covalent bond formation. In this study, we have synthesized PNAs incorporating 4-amino-6-oxo-2-vinylpyrimidine (AOVP) as a crosslinking agent and evaluated their reactivities for targeting DNA and RNA.",

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AU - Akisawa, Takuya

AU - Ishizawa, Yuki

AU - Nagatsugi, Fumi

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AB - Peptide nucleic acids (PNAs) are structural mimics of nucleic acids that form stable hybrids with DNA and RNA. In addition, PNAs can invade double-stranded DNA. Due to these characteristics, PNAs are widely used as biochemical tools, for example, in antisense/antigene therapy. Interstrand crosslink formation in nucleic acids is one of the strategies for preparing a stable duplex by covalent bond formation. In this study, we have synthesized PNAs incorporating 4-amino-6-oxo-2-vinylpyrimidine (AOVP) as a crosslinking agent and evaluated their reactivities for targeting DNA and RNA.

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KW - Crosslink

KW - Invasion

KW - Peptide nucleic acid (PNA)

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Synthesis of peptide nucleic acids containing a crosslinking agent and evaluation of their reactivities

Abstract

Keywords

ASJC Scopus subject areas

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