Synthesis of photoaffinity probe based on the leaf-opening factor from genus Albizzia

Masahiro Okada; Akira Matsubara; Minoru Ueda

doi:10.1016/j.tetlet.2008.04.002

Synthesis of photoaffinity probe based on the leaf-opening factor from genus Albizzia

Masahiro Okada, Akira Matsubara, Minoru Ueda

SCI - Chemistry

Tohoku University

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

The circadian rhythmic leaf-movements in legumes, called nyctinasty, are regulated by a pair of leaf-closing and -opening factors. Recent fluorescence studies revealed that cis-p-coumaroylagmatine (1), the leaf-opening factor from genus Albizzia, specifically bound to motor cells in a joint of leaf and stem. In order to identify the receptor protein, which is expected to be involved in the plasma membrane of the cell, we designed and synthesized a photoaffinity probe (2), containing a photoreactive azide group on the aromatic ring and FLAG segment as a peptide antigen for immunoprecipitation.

Original language	English
Pages (from-to)	3794-3796
Number of pages	3
Journal	Tetrahedron Letters
Volume	49
Issue number	23
DOIs	https://doi.org/10.1016/j.tetlet.2008.04.002
Publication status	Published - 2008 Jun 2

Keywords

Albizzia saman
Leaf-opening factor
Nyctinasty
Photoaffinity probe

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10.1016/j.tetlet.2008.04.002

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title = "Synthesis of photoaffinity probe based on the leaf-opening factor from genus Albizzia",

abstract = "The circadian rhythmic leaf-movements in legumes, called nyctinasty, are regulated by a pair of leaf-closing and -opening factors. Recent fluorescence studies revealed that cis-p-coumaroylagmatine (1), the leaf-opening factor from genus Albizzia, specifically bound to motor cells in a joint of leaf and stem. In order to identify the receptor protein, which is expected to be involved in the plasma membrane of the cell, we designed and synthesized a photoaffinity probe (2), containing a photoreactive azide group on the aromatic ring and FLAG segment as a peptide antigen for immunoprecipitation.",

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author = "Masahiro Okada and Akira Matsubara and Minoru Ueda",

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AU - Okada, Masahiro

AU - Matsubara, Akira

AU - Ueda, Minoru

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Y1 - 2008/6/2

N2 - The circadian rhythmic leaf-movements in legumes, called nyctinasty, are regulated by a pair of leaf-closing and -opening factors. Recent fluorescence studies revealed that cis-p-coumaroylagmatine (1), the leaf-opening factor from genus Albizzia, specifically bound to motor cells in a joint of leaf and stem. In order to identify the receptor protein, which is expected to be involved in the plasma membrane of the cell, we designed and synthesized a photoaffinity probe (2), containing a photoreactive azide group on the aromatic ring and FLAG segment as a peptide antigen for immunoprecipitation.

AB - The circadian rhythmic leaf-movements in legumes, called nyctinasty, are regulated by a pair of leaf-closing and -opening factors. Recent fluorescence studies revealed that cis-p-coumaroylagmatine (1), the leaf-opening factor from genus Albizzia, specifically bound to motor cells in a joint of leaf and stem. In order to identify the receptor protein, which is expected to be involved in the plasma membrane of the cell, we designed and synthesized a photoaffinity probe (2), containing a photoreactive azide group on the aromatic ring and FLAG segment as a peptide antigen for immunoprecipitation.

KW - Albizzia saman

KW - Leaf-opening factor

KW - Nyctinasty

KW - Photoaffinity probe

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JO - Tetrahedron Letters

JF - Tetrahedron Letters

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ER -

Synthesis of photoaffinity probe based on the leaf-opening factor from genus Albizzia

Abstract

Keywords

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