Synthesis on aggregation of colloidal solutions of ICG-active silica nanoparticles and their application in in‐vivo fluorescence imaging

Yuta Shindo; Tomoya Inose; Yohsuke Kubota; Takahiro Oikawa; Masayuki Tokunaga; Takashi Kamei; Kohsuke Gonda; Yoshio Kobayashi

doi:10.1016/j.matchemphys.2018.08.073

Synthesis on aggregation of colloidal solutions of ICG-active silica nanoparticles and their application in in‐vivo fluorescence imaging

Yuta Shindo, Tomoya Inose, Yohsuke Kubota, Takahiro Oikawa, Masayuki Tokunaga, Takashi Kamei, Kohsuke Gonda, Yoshio Kobayashi

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3 Citations (Scopus)

Abstract

This study reports two findings. The first is a method for preparing a colloidal solution of silica (SiO₂) nanoparticles activated with fluorescent dye or fluorescent dye-active SiO₂ nanoparticles. Preparation of a colloid solution of spherical SiO₂ nanoparticles with an average size of 109.4 ± 8.6 nm was performed using the sol-gel method at 35 °C using 0.2 M tetraethylorthosilicate, 25 M H₂O, and 0.01 M NaOH in ethanol. Two kinds of cationic chemicals (poly-diallyldimethylammonium chloride (PDADMAC) and (3-aminopropyl) triethoxysilane (APES) were examined for surface-modification or surface-cationization of the SiO₂ nanoparticles (SiO₂/PDADMAC and SiO₂/APES). To activate the SiO₂ nanoparticles with fluorescent dye, indocyanine green (ICG) molecules were fixed on the surface-modified SiO₂ nanoparticles by mixing ICG dissolved in albumin aqueous solution and the surface-modified SiO₂ nanoparticle colloid solution (SiO₂/PDADMAC/ICG and SiO₂/APES/ICG) through electrostatic aggregation. The second finding is the verification of the fluorescence ability of the ICG-active SiO₂ nanoparticle colloid solutions. Both the ICG-active SiO₂ nanoparticle colloid solutions exerted fluorescence even at high ICG concentrations. Mouse tissues could be imaged by injecting the ICG-immobilized SiO₂ nanoparticle colloid solution into the tail vein of the mouse and measuring the emitted fluorescence intensity. Pulmonary embolism was avoided in the SiO₂/APES/ICG particle colloid solution-injected group, in which the particles reached the tissues more efficiently than in the SiO₂/PDADMAC/ICG nanoparticles-injected group.

Original language	English
Pages (from-to)	201-207
Number of pages	7
Journal	Materials Chemistry and Physics
Volume	220
DOIs	https://doi.org/10.1016/j.matchemphys.2018.08.073
Publication status	Published - 2018 Dec 1

Keywords

Fluorescent marker
Indocyanine green
Nanoparticles
Silica
Sol-gel

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10.1016/j.matchemphys.2018.08.073

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@article{eccbee9e4ec647a5be1121437d7fe611,

title = "Synthesis on aggregation of colloidal solutions of ICG-active silica nanoparticles and their application in in‐vivo fluorescence imaging",

abstract = "This study reports two findings. The first is a method for preparing a colloidal solution of silica (SiO2) nanoparticles activated with fluorescent dye or fluorescent dye-active SiO2 nanoparticles. Preparation of a colloid solution of spherical SiO2 nanoparticles with an average size of 109.4 ± 8.6 nm was performed using the sol-gel method at 35 °C using 0.2 M tetraethylorthosilicate, 25 M H2O, and 0.01 M NaOH in ethanol. Two kinds of cationic chemicals (poly-diallyldimethylammonium chloride (PDADMAC) and (3-aminopropyl) triethoxysilane (APES) were examined for surface-modification or surface-cationization of the SiO2 nanoparticles (SiO2/PDADMAC and SiO2/APES). To activate the SiO2 nanoparticles with fluorescent dye, indocyanine green (ICG) molecules were fixed on the surface-modified SiO2 nanoparticles by mixing ICG dissolved in albumin aqueous solution and the surface-modified SiO2 nanoparticle colloid solution (SiO2/PDADMAC/ICG and SiO2/APES/ICG) through electrostatic aggregation. The second finding is the verification of the fluorescence ability of the ICG-active SiO2 nanoparticle colloid solutions. Both the ICG-active SiO2 nanoparticle colloid solutions exerted fluorescence even at high ICG concentrations. Mouse tissues could be imaged by injecting the ICG-immobilized SiO2 nanoparticle colloid solution into the tail vein of the mouse and measuring the emitted fluorescence intensity. Pulmonary embolism was avoided in the SiO2/APES/ICG particle colloid solution-injected group, in which the particles reached the tissues more efficiently than in the SiO2/PDADMAC/ICG nanoparticles-injected group.",

keywords = "Fluorescent marker, Indocyanine green, Nanoparticles, Silica, Sol-gel",

author = "Yuta Shindo and Tomoya Inose and Yohsuke Kubota and Takahiro Oikawa and Masayuki Tokunaga and Takashi Kamei and Kohsuke Gonda and Yoshio Kobayashi",

note = "Funding Information: A portion of this work was supported by a Grant-in-Aid for Scientific Research (B) ( 16H05168 ) and Exploratory Research ( 16K15572 ) from the Japan Society for the Promotion of Science (JSPS). We express our thanks to Prof. T. Noguchi of the College of Science of Ibaraki University, Japan (current affiliation: Faculty of Arts and Science of Kyusyu University, Japan) for his assistance with the TEM observations. Publisher Copyright: {\textcopyright} 2018 Elsevier B.V.",

year = "2018",

month = dec,

day = "1",

doi = "10.1016/j.matchemphys.2018.08.073",

language = "English",

volume = "220",

pages = "201--207",

journal = "Materials Chemistry and Physics",

issn = "0254-0584",

publisher = "Elsevier BV",

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T1 - Synthesis on aggregation of colloidal solutions of ICG-active silica nanoparticles and their application in in‐vivo fluorescence imaging

AU - Shindo, Yuta

AU - Inose, Tomoya

AU - Kubota, Yohsuke

AU - Oikawa, Takahiro

AU - Tokunaga, Masayuki

AU - Kamei, Takashi

AU - Gonda, Kohsuke

AU - Kobayashi, Yoshio

N1 - Funding Information: A portion of this work was supported by a Grant-in-Aid for Scientific Research (B) ( 16H05168 ) and Exploratory Research ( 16K15572 ) from the Japan Society for the Promotion of Science (JSPS). We express our thanks to Prof. T. Noguchi of the College of Science of Ibaraki University, Japan (current affiliation: Faculty of Arts and Science of Kyusyu University, Japan) for his assistance with the TEM observations. Publisher Copyright: © 2018 Elsevier B.V.

PY - 2018/12/1

Y1 - 2018/12/1

N2 - This study reports two findings. The first is a method for preparing a colloidal solution of silica (SiO2) nanoparticles activated with fluorescent dye or fluorescent dye-active SiO2 nanoparticles. Preparation of a colloid solution of spherical SiO2 nanoparticles with an average size of 109.4 ± 8.6 nm was performed using the sol-gel method at 35 °C using 0.2 M tetraethylorthosilicate, 25 M H2O, and 0.01 M NaOH in ethanol. Two kinds of cationic chemicals (poly-diallyldimethylammonium chloride (PDADMAC) and (3-aminopropyl) triethoxysilane (APES) were examined for surface-modification or surface-cationization of the SiO2 nanoparticles (SiO2/PDADMAC and SiO2/APES). To activate the SiO2 nanoparticles with fluorescent dye, indocyanine green (ICG) molecules were fixed on the surface-modified SiO2 nanoparticles by mixing ICG dissolved in albumin aqueous solution and the surface-modified SiO2 nanoparticle colloid solution (SiO2/PDADMAC/ICG and SiO2/APES/ICG) through electrostatic aggregation. The second finding is the verification of the fluorescence ability of the ICG-active SiO2 nanoparticle colloid solutions. Both the ICG-active SiO2 nanoparticle colloid solutions exerted fluorescence even at high ICG concentrations. Mouse tissues could be imaged by injecting the ICG-immobilized SiO2 nanoparticle colloid solution into the tail vein of the mouse and measuring the emitted fluorescence intensity. Pulmonary embolism was avoided in the SiO2/APES/ICG particle colloid solution-injected group, in which the particles reached the tissues more efficiently than in the SiO2/PDADMAC/ICG nanoparticles-injected group.

AB - This study reports two findings. The first is a method for preparing a colloidal solution of silica (SiO2) nanoparticles activated with fluorescent dye or fluorescent dye-active SiO2 nanoparticles. Preparation of a colloid solution of spherical SiO2 nanoparticles with an average size of 109.4 ± 8.6 nm was performed using the sol-gel method at 35 °C using 0.2 M tetraethylorthosilicate, 25 M H2O, and 0.01 M NaOH in ethanol. Two kinds of cationic chemicals (poly-diallyldimethylammonium chloride (PDADMAC) and (3-aminopropyl) triethoxysilane (APES) were examined for surface-modification or surface-cationization of the SiO2 nanoparticles (SiO2/PDADMAC and SiO2/APES). To activate the SiO2 nanoparticles with fluorescent dye, indocyanine green (ICG) molecules were fixed on the surface-modified SiO2 nanoparticles by mixing ICG dissolved in albumin aqueous solution and the surface-modified SiO2 nanoparticle colloid solution (SiO2/PDADMAC/ICG and SiO2/APES/ICG) through electrostatic aggregation. The second finding is the verification of the fluorescence ability of the ICG-active SiO2 nanoparticle colloid solutions. Both the ICG-active SiO2 nanoparticle colloid solutions exerted fluorescence even at high ICG concentrations. Mouse tissues could be imaged by injecting the ICG-immobilized SiO2 nanoparticle colloid solution into the tail vein of the mouse and measuring the emitted fluorescence intensity. Pulmonary embolism was avoided in the SiO2/APES/ICG particle colloid solution-injected group, in which the particles reached the tissues more efficiently than in the SiO2/PDADMAC/ICG nanoparticles-injected group.

KW - Fluorescent marker

KW - Indocyanine green

KW - Nanoparticles

KW - Silica

KW - Sol-gel

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U2 - 10.1016/j.matchemphys.2018.08.073

DO - 10.1016/j.matchemphys.2018.08.073

M3 - Article

AN - SCOPUS:85052614654

SN - 0254-0584

VL - 220

SP - 201

EP - 207

JO - Materials Chemistry and Physics

JF - Materials Chemistry and Physics

ER -

Synthesis on aggregation of colloidal solutions of ICG-active silica nanoparticles and their application in in‐vivo fluorescence imaging

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Keywords

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