@article{a71b06e459ea4e09b0586b9e6c2c2c21,
title = "Synthetic-biology-based discovery of a fungal macrolide from: Macrophomina phaseolina",
abstract = "A synthetic biology approach based on genome mining and heterologous biosynthesis is a powerful tool for discovering novel natural products from a tremendous gene resource. We carried out fungal genome mining guided by a polyketide synthase gene using a public database and found a putative macrolide biosynthetic gene cluster with a highly reducing polyketide synthase gene and a thioesterase gene in Macrophomina phaseolina. Reconstitution of the cluster in Aspergillus oryzae, a model heterologous host for fungal natural product biosynthesis, produced a new 12-membered macrolide, phaseolide A. The absolute stereochemistry was elucidated by vibrational circular dichroism spectroscopy and the crystalline sponge method.",
author = "Yohei Morishita and Terutaka Sonohara and Tohru Taniguchi and Kiyohiro Adachi and Makoto Fujita and Teigo Asai",
note = "Funding Information: We thank Prof. K. Gomi (Tohoku University) and Prof. K. Kitamoto (the University of Tokyo) for providing the expression vectors and the fungal strain. This work was supported by JSPS KAKENHI (grant no. 17H05055, 17H05428 and 19H04642 to T. Asai from the Japan Society for the Promotion of Science (JSPS)), the Naito Foundation and the Astellas Foundation for Research on Metabolic Disorders. A part of this work was conducted in the Institute for Molecular Science, supported by Nanotechnology Platform Program (Molecule and Material Synthesis) of the Ministry of Education, Culture, Sports, Science and Technology (MEXT), Japan. We are grateful to the Equipment Development Center, Institute for Molecular Science, for assistance. Publisher Copyright: This journal is {\textcopyright} The Royal Society of Chemistry.",
year = "2020",
month = apr,
day = "21",
doi = "10.1039/d0ob00519c",
language = "English",
volume = "18",
pages = "2813--2816",
journal = "Organic and Biomolecular Chemistry",
issn = "1477-0520",
publisher = "Royal Society of Chemistry",
number = "15",
}