TY - JOUR
T1 - Systematic characterization of dmpc/dhpc self-assemblies and their phase behaviors in aqueous solution
AU - Taguchi, Shogo
AU - Suga, Keishi
AU - Hayashi, Keita
AU - Okamoto, Yukihiro
AU - Jung, Ho Sup
AU - Nakamura, Hidemi
AU - Umakoshi, Hiroshi
N1 - Funding Information:
Funding: This work was supported by the Japan Society for the Promotion of Science (JSPS) KAKENHI Grant-in-Aids for Scientific Research (A) (26249116), for Scientific Research (B) (18H02005), for Scientific Research (C) (26420771), for Young Scientists (B) (16K18279, 15K18279), and for Challenging Exploratory Research (T15K142040). And supported by the Grant-in-Aid for the Sasakawa Scientific Research Grant from the Japan Science Society, JSS (29-218).
Publisher Copyright:
© 2018 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2018/12
Y1 - 2018/12
N2 - Self-assemblies composed of 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) and 1,2-dihexanoyl-sn-glycero-3-phosphocholine (DHPC) form several kinds of structures, such as vesicle, micelle, and bicelle. Their morphological properties have been studied widely, but their interfacial membrane properties have not been adequately investigated. Herein, we report a systematic characterization of DMPC/DHPC assemblies at 20◦C. To investigate the phase behavior, optical density OD500, size (by dynamic light scattering), membrane fluidity 1/PDPH (using 1,6-diphenyl-1,3,5-hexatriene), and membrane polarity GP340 (using 6-dodecanoyl-N,N-dimethyl-2-naphthylamine) were measured as a function of molar ratio of DHPC (XDHPC). Based on structural properties (OD500 and size), large and small assemblies were categorized into Region (i) (XDHPC < 0.4) and Region (ii) (XDHPC ≥ 0.4), respectively. The DMPC/DHPC assemblies with 0.33 ≤ XDHPC ≤ 0.67 (Region (ii-1)) showed gel-phase-like interfacial membrane properties, whereas DHPC-rich assemblies (XDHPC ≥ 0.77) showed disordered membrane properties (Region (ii-2)). Considering the structural and interfacial membrane properties, the DMPC/DHPC assemblies in Regions (i), (ii-1), and (ii-2) can be determined to be vesicle, bicelle, and micelle, respectively.
AB - Self-assemblies composed of 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) and 1,2-dihexanoyl-sn-glycero-3-phosphocholine (DHPC) form several kinds of structures, such as vesicle, micelle, and bicelle. Their morphological properties have been studied widely, but their interfacial membrane properties have not been adequately investigated. Herein, we report a systematic characterization of DMPC/DHPC assemblies at 20◦C. To investigate the phase behavior, optical density OD500, size (by dynamic light scattering), membrane fluidity 1/PDPH (using 1,6-diphenyl-1,3,5-hexatriene), and membrane polarity GP340 (using 6-dodecanoyl-N,N-dimethyl-2-naphthylamine) were measured as a function of molar ratio of DHPC (XDHPC). Based on structural properties (OD500 and size), large and small assemblies were categorized into Region (i) (XDHPC < 0.4) and Region (ii) (XDHPC ≥ 0.4), respectively. The DMPC/DHPC assemblies with 0.33 ≤ XDHPC ≤ 0.67 (Region (ii-1)) showed gel-phase-like interfacial membrane properties, whereas DHPC-rich assemblies (XDHPC ≥ 0.77) showed disordered membrane properties (Region (ii-2)). Considering the structural and interfacial membrane properties, the DMPC/DHPC assemblies in Regions (i), (ii-1), and (ii-2) can be determined to be vesicle, bicelle, and micelle, respectively.
KW - Bicelle
KW - Membrane fluidity
KW - Membrane polarity
KW - Phase behavior
KW - Phospholipid assembly
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U2 - 10.3390/colloids2040073
DO - 10.3390/colloids2040073
M3 - Article
AN - SCOPUS:85071258882
SN - 2504-5377
VL - 2
JO - Colloids and Interfaces
JF - Colloids and Interfaces
IS - 4
M1 - 73
ER -