This paper focuses on controlling adsorption of gold nanoparticles onto polymer nanosheets. Amphiphilic copolymers, poly(N-dodecyl acrylamide-co-4-vinyl pyridine)s (p(DDA/VPy)s), were synthesized and the ultrathin p(DDA/VPy) films were prepared by the Langmuir-Blodgett (LB) technique. Gold nanoparticles were immobilized onto p(DDA/VPy) LB films through the dipping method and took a uniformly distributed monolayer formation. The amount of gold nanopariticle in the monolayer strongly depended on the VPy content. A patterned gold nanoparticle monolayer was obtained with photopatterned p(DDA/VPy) LB films. These findings suggest that p(DDA/VPy) LB films act as a good template for gold nanoparticle arrays.