TALEN-mediated mutagenesis in zebrafish reveals a role for r-spondin 2 in fin ray and vertebral development

Yoshiaki Tatsumi; Moe Takeda; Masaru Matsuda; Tohru Suzuki; Hayato Yokoi

doi:10.1016/j.febslet.2014.10.015

TALEN-mediated mutagenesis in zebrafish reveals a role for r-spondin 2 in fin ray and vertebral development

Yoshiaki Tatsumi, Moe Takeda, Masaru Matsuda, Tohru Suzuki, Hayato Yokoi

AGR - Agricultural Bioscience

Research output: Contribution to journal › Article › peer-review

20 Citations (Scopus)

Abstract

R-spondin (Rspo) encodes a multi-domain protein that modulates the Wnt-signaling pathway. Two distinct rspo2 zebrafish mutants were generated by TALEN-mediated mutagenesis: a null mutant, rspo2^null, lacking all functional domains, and a hypomorphic mutant, rspo2^tsp, lacking the two N-terminal domains. Mutants were analyzed mainly for abnormalities in the skeletal system. Fin ray skeletons were formed normally in the rspo2^tsp mutants, but were absent from the rspo2^null mutants. Hypoplasia of the neural/hemal arches and ribs was observed in both mutants. Thus, the two rspo2 mutants help to identify the functions of Rspo2 in skeletogenesis, as well as functional differences among multiple Rspo2 domains.

Original language	English
Pages (from-to)	4543-4550
Number of pages	8
Journal	FEBS Letters
Volume	588
Issue number	24
DOIs	https://doi.org/10.1016/j.febslet.2014.10.015
Publication status	Published - 2014 Dec 20

Keywords

Fin ray
Mutagenesis
R-spondin2
Skeletogenesis
TALEN
Zebrafish

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10.1016/j.febslet.2014.10.015

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title = "TALEN-mediated mutagenesis in zebrafish reveals a role for r-spondin 2 in fin ray and vertebral development",

abstract = "R-spondin (Rspo) encodes a multi-domain protein that modulates the Wnt-signaling pathway. Two distinct rspo2 zebrafish mutants were generated by TALEN-mediated mutagenesis: a null mutant, rspo2null, lacking all functional domains, and a hypomorphic mutant, rspo2tsp, lacking the two N-terminal domains. Mutants were analyzed mainly for abnormalities in the skeletal system. Fin ray skeletons were formed normally in the rspo2tsp mutants, but were absent from the rspo2null mutants. Hypoplasia of the neural/hemal arches and ribs was observed in both mutants. Thus, the two rspo2 mutants help to identify the functions of Rspo2 in skeletogenesis, as well as functional differences among multiple Rspo2 domains.",

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author = "Yoshiaki Tatsumi and Moe Takeda and Masaru Matsuda and Tohru Suzuki and Hayato Yokoi",

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T1 - TALEN-mediated mutagenesis in zebrafish reveals a role for r-spondin 2 in fin ray and vertebral development

AU - Tatsumi, Yoshiaki

AU - Takeda, Moe

AU - Matsuda, Masaru

AU - Suzuki, Tohru

AU - Yokoi, Hayato

PY - 2014/12/20

Y1 - 2014/12/20

N2 - R-spondin (Rspo) encodes a multi-domain protein that modulates the Wnt-signaling pathway. Two distinct rspo2 zebrafish mutants were generated by TALEN-mediated mutagenesis: a null mutant, rspo2null, lacking all functional domains, and a hypomorphic mutant, rspo2tsp, lacking the two N-terminal domains. Mutants were analyzed mainly for abnormalities in the skeletal system. Fin ray skeletons were formed normally in the rspo2tsp mutants, but were absent from the rspo2null mutants. Hypoplasia of the neural/hemal arches and ribs was observed in both mutants. Thus, the two rspo2 mutants help to identify the functions of Rspo2 in skeletogenesis, as well as functional differences among multiple Rspo2 domains.

AB - R-spondin (Rspo) encodes a multi-domain protein that modulates the Wnt-signaling pathway. Two distinct rspo2 zebrafish mutants were generated by TALEN-mediated mutagenesis: a null mutant, rspo2null, lacking all functional domains, and a hypomorphic mutant, rspo2tsp, lacking the two N-terminal domains. Mutants were analyzed mainly for abnormalities in the skeletal system. Fin ray skeletons were formed normally in the rspo2tsp mutants, but were absent from the rspo2null mutants. Hypoplasia of the neural/hemal arches and ribs was observed in both mutants. Thus, the two rspo2 mutants help to identify the functions of Rspo2 in skeletogenesis, as well as functional differences among multiple Rspo2 domains.

KW - Fin ray

KW - Mutagenesis

KW - R-spondin2

KW - Skeletogenesis

KW - TALEN

KW - Zebrafish

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TALEN-mediated mutagenesis in zebrafish reveals a role for r-spondin 2 in fin ray and vertebral development

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Keywords

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