Tapetum-specific expression of the Osg6B promoter-β-glucuronidase gene in transgenic rice

S. Yokoi, T. Tsuchiya, K. Toriyama, K. Hinata

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59 Citations (Scopus)

Abstract

The promoter of an anther tapetum-specific gene, Osg6B, was fused to a β-glucuronidase (GUS) gene and introduced into rice by Agrobacterium-mediated gene transfer. Fluorometric and histochemical GUS assay showed that GUS was expressed exclusively within the tapetum of anthers from the uninucleate microspore stage (7 days before anthesis) to the tricellular pollen stage (3 days before anthesis). This is the first demonstration of an anther-specific promoter directing tapetum-specific expression in rice.

Original languageEnglish
Pages (from-to)363-367
Number of pages5
JournalPlant Cell Reports
Volume16
Issue number6
DOIs
Publication statusPublished - 1997 Mar

Keywords

  • Agrobacterium
  • Rice
  • Tapetum-specific promoter
  • Transformation

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