Targeted disruption of the mouse protein phosphatase ppm1l gene leads to structural abnormalities in the brain

Rie Kusano; Kousuke Fujita; Yasuharu Shinoda; Yuko Nagaura; Hiroshi Kiyonari; Takaya Abe; Toshio Watanabe; Yasuhisa Matsui; Masahiro Fukaya; Hiroyuki Sakagami; Tatsuya Sato; Jun Ichi Funahashi; Motoko Ohnishi; Shinri Tamura; Takayasu Kobayashi

doi:10.1002/1873-3468.12429

Targeted disruption of the mouse protein phosphatase ppm1l gene leads to structural abnormalities in the brain

Rie Kusano, Kousuke Fujita, Yasuharu Shinoda, Yuko Nagaura, Hiroshi Kiyonari, Takaya Abe, Toshio Watanabe, Yasuhisa Matsui, Masahiro Fukaya, Hiroyuki Sakagami, Tatsuya Sato, Jun Ichi Funahashi, Motoko Ohnishi, Shinri Tamura, Takayasu Kobayashi

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

PPM1L, a member of the metal-dependent protein phosphatase (PPM) family, is involved in regulating the stress-activated protein kinase pathway and ceramide trafficking. However, the physiological function of PPM1L in the brain is unclear. In this study, we generated and analyzed ppm1l-deficient mice in order to investigate PPM1L functions in the brain. Our results indicate that ppm1l is highly expressed in the central nervous system during mouse development and that ppm1l^Δ/Δ mice display impaired motor performance and morphological abnormalities in the forebrain. Electron microscopic and immunohistochemical analyses suggest that these abnormalities are due to impaired axonal tract formation. Our novel findings suggest an important role for PPM1L in brain development.

Original language	English
Pages (from-to)	3606-3615
Number of pages	10
Journal	FEBS Letters
Volume	590
Issue number	20
DOIs	https://doi.org/10.1002/1873-3468.12429
Publication status	Published - 2016 Oct 1

Keywords

MAPK signaling
brain development
commissural tract
ppm1l
protein phosphatase
striatum

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10.1002/1873-3468.12429

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Kusano, R., Fujita, K., Shinoda, Y., Nagaura, Y., Kiyonari, H., Abe, T., Watanabe, T., Matsui, Y., Fukaya, M., Sakagami, H., Sato, T., Funahashi, J. I., Ohnishi, M., Tamura, S., & Kobayashi, T. (2016). Targeted disruption of the mouse protein phosphatase ppm1l gene leads to structural abnormalities in the brain. FEBS Letters, 590(20), 3606-3615. https://doi.org/10.1002/1873-3468.12429

@article{1f430db548ae4fc0ac686eb390691e4c,

title = "Targeted disruption of the mouse protein phosphatase ppm1l gene leads to structural abnormalities in the brain",

abstract = "PPM1L, a member of the metal-dependent protein phosphatase (PPM) family, is involved in regulating the stress-activated protein kinase pathway and ceramide trafficking. However, the physiological function of PPM1L in the brain is unclear. In this study, we generated and analyzed ppm1l-deficient mice in order to investigate PPM1L functions in the brain. Our results indicate that ppm1l is highly expressed in the central nervous system during mouse development and that ppm1lΔ/Δ mice display impaired motor performance and morphological abnormalities in the forebrain. Electron microscopic and immunohistochemical analyses suggest that these abnormalities are due to impaired axonal tract formation. Our novel findings suggest an important role for PPM1L in brain development.",

keywords = "MAPK signaling, brain development, commissural tract, ppm1l, protein phosphatase, striatum",

author = "Rie Kusano and Kousuke Fujita and Yasuharu Shinoda and Yuko Nagaura and Hiroshi Kiyonari and Takaya Abe and Toshio Watanabe and Yasuhisa Matsui and Masahiro Fukaya and Hiroyuki Sakagami and Tatsuya Sato and Funahashi, {Jun Ichi} and Motoko Ohnishi and Shinri Tamura and Takayasu Kobayashi",

note = "Publisher Copyright: {\textcopyright} 2016 Federation of European Biochemical Societies",

year = "2016",

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day = "1",

doi = "10.1002/1873-3468.12429",

language = "English",

volume = "590",

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Kusano, R, Fujita, K, Shinoda, Y, Nagaura, Y, Kiyonari, H, Abe, T, Watanabe, T, Matsui, Y, Fukaya, M, Sakagami, H, Sato, T, Funahashi, JI, Ohnishi, M, Tamura, S & Kobayashi, T 2016, 'Targeted disruption of the mouse protein phosphatase ppm1l gene leads to structural abnormalities in the brain', FEBS Letters, vol. 590, no. 20, pp. 3606-3615. https://doi.org/10.1002/1873-3468.12429

TY - JOUR

T1 - Targeted disruption of the mouse protein phosphatase ppm1l gene leads to structural abnormalities in the brain

AU - Kusano, Rie

AU - Fujita, Kousuke

AU - Shinoda, Yasuharu

AU - Nagaura, Yuko

AU - Kiyonari, Hiroshi

AU - Abe, Takaya

AU - Watanabe, Toshio

AU - Matsui, Yasuhisa

AU - Fukaya, Masahiro

AU - Sakagami, Hiroyuki

AU - Sato, Tatsuya

AU - Funahashi, Jun Ichi

AU - Ohnishi, Motoko

AU - Tamura, Shinri

AU - Kobayashi, Takayasu

PY - 2016/10/1

Y1 - 2016/10/1

N2 - PPM1L, a member of the metal-dependent protein phosphatase (PPM) family, is involved in regulating the stress-activated protein kinase pathway and ceramide trafficking. However, the physiological function of PPM1L in the brain is unclear. In this study, we generated and analyzed ppm1l-deficient mice in order to investigate PPM1L functions in the brain. Our results indicate that ppm1l is highly expressed in the central nervous system during mouse development and that ppm1lΔ/Δ mice display impaired motor performance and morphological abnormalities in the forebrain. Electron microscopic and immunohistochemical analyses suggest that these abnormalities are due to impaired axonal tract formation. Our novel findings suggest an important role for PPM1L in brain development.

AB - PPM1L, a member of the metal-dependent protein phosphatase (PPM) family, is involved in regulating the stress-activated protein kinase pathway and ceramide trafficking. However, the physiological function of PPM1L in the brain is unclear. In this study, we generated and analyzed ppm1l-deficient mice in order to investigate PPM1L functions in the brain. Our results indicate that ppm1l is highly expressed in the central nervous system during mouse development and that ppm1lΔ/Δ mice display impaired motor performance and morphological abnormalities in the forebrain. Electron microscopic and immunohistochemical analyses suggest that these abnormalities are due to impaired axonal tract formation. Our novel findings suggest an important role for PPM1L in brain development.

KW - MAPK signaling

KW - brain development

KW - commissural tract

KW - ppm1l

KW - protein phosphatase

KW - striatum

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EP - 3615

JO - FEBS Letters

JF - FEBS Letters

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ER -

Targeted disruption of the mouse protein phosphatase ppm1l gene leads to structural abnormalities in the brain

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