Targeted expression of IP3 sponge and IP3 DsRNA impaires sugar taste sensation in Drosophila

Kazue Usui-Aoki; Ken Matsumoto; Masayuki Koganezawa; Sou Kohatsu; Kunio Isono; Hiroshi Matsubayashi; Masa Toshi Yamamoto; Ryu Ueda; Kuniaki Takahashi; Kaoru Saigo; Katsuhiko Mikoshiba; Daisuke Yamamoto

doi:10.1080/01677060600569713

Targeted expression of IP₃ sponge and IP₃ DsRNA impaires sugar taste sensation in Drosophila

Kazue Usui-Aoki, Ken Matsumoto, Masayuki Koganezawa, Sou Kohatsu, Kunio Isono, Hiroshi Matsubayashi, Masa Toshi Yamamoto, Ryu Ueda, Kuniaki Takahashi, Kaoru Saigo, Katsuhiko Mikoshiba, Daisuke Yamamoto

LIF - Integrative Life Sciences

Research output: Contribution to journal › Article › peer-review

23 Citations (Scopus)

Abstract

We evaluated the role of IP₃ in sugar taste reception in Drosophila melanogaster by inactivating the IP₃ signaling using genetic tools. We used the "IP₃ sponge," composed of the modified ligand-binding domain from the mouse IP₃ receptor, which was designed to absorb IP₃ in competition with native IP₃ receptors. Another tool was a transgene that generates double-stranded RNA against IP₃ receptor mRNA. Both inhibitors diminished the sensitivity of flies to trehalose and sucrose, as estimated by behavioral assays and electrophysiological recordings from the sugar receptor cells. The result indicates that IP₃ signaling is indispensable for sugar reception in Drosophila.

Original language	English
Pages (from-to)	123-141
Number of pages	19
Journal	Journal of neurogenetics
Volume	19
Issue number	3-4
DOIs	https://doi.org/10.1080/01677060600569713
Publication status	Published - 2005 Jul

Keywords

Behavioral responses
Feeding
Gal4 transgene
IP
Inhibitory RNA
Sugar sensation

ASJC Scopus subject areas

Genetics
Cellular and Molecular Neuroscience

Access to Document

10.1080/01677060600569713

Cite this

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title = "Targeted expression of IP3 sponge and IP3 DsRNA impaires sugar taste sensation in Drosophila",

abstract = "We evaluated the role of IP3 in sugar taste reception in Drosophila melanogaster by inactivating the IP3 signaling using genetic tools. We used the {"}IP3 sponge,{"} composed of the modified ligand-binding domain from the mouse IP3 receptor, which was designed to absorb IP3 in competition with native IP3 receptors. Another tool was a transgene that generates double-stranded RNA against IP3 receptor mRNA. Both inhibitors diminished the sensitivity of flies to trehalose and sucrose, as estimated by behavioral assays and electrophysiological recordings from the sugar receptor cells. The result indicates that IP3 signaling is indispensable for sugar reception in Drosophila.",

keywords = "Behavioral responses, Feeding, Gal4 transgene, IP, Inhibitory RNA, Sugar sensation",

author = "Kazue Usui-Aoki and Ken Matsumoto and Masayuki Koganezawa and Sou Kohatsu and Kunio Isono and Hiroshi Matsubayashi and Yamamoto, {Masa Toshi} and Ryu Ueda and Kuniaki Takahashi and Kaoru Saigo and Katsuhiko Mikoshiba and Daisuke Yamamoto",

note = "Funding Information: We thank Takuma Takanashi for his involvement in some preliminary experiments and K.-I. Kimura, E. Suzuki and the Bloomington Stock Center for fly stocks. This work was supported by Special Coordination Funds for Promoting Science and Technology from the MinistryofEducation,Culture,Sports,andScienceandTechnology(MEXT),and {\textquoteleft}{\textquoteleft}Academic Frontier{\textquoteright}{\textquoteright} Project for Private Universities from MEXT, 2003–2008 to Daisuke Yamamoto.",

year = "2005",

month = jul,

doi = "10.1080/01677060600569713",

language = "English",

volume = "19",

pages = "123--141",

journal = "Journal of Neurogenetics",

issn = "0167-7063",

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T1 - Targeted expression of IP3 sponge and IP3 DsRNA impaires sugar taste sensation in Drosophila

AU - Usui-Aoki, Kazue

AU - Matsumoto, Ken

AU - Koganezawa, Masayuki

AU - Kohatsu, Sou

AU - Isono, Kunio

AU - Matsubayashi, Hiroshi

AU - Yamamoto, Masa Toshi

AU - Ueda, Ryu

AU - Takahashi, Kuniaki

AU - Saigo, Kaoru

AU - Mikoshiba, Katsuhiko

AU - Yamamoto, Daisuke

N1 - Funding Information: We thank Takuma Takanashi for his involvement in some preliminary experiments and K.-I. Kimura, E. Suzuki and the Bloomington Stock Center for fly stocks. This work was supported by Special Coordination Funds for Promoting Science and Technology from the MinistryofEducation,Culture,Sports,andScienceandTechnology(MEXT),and ‘‘Academic Frontier’’ Project for Private Universities from MEXT, 2003–2008 to Daisuke Yamamoto.

PY - 2005/7

Y1 - 2005/7

N2 - We evaluated the role of IP3 in sugar taste reception in Drosophila melanogaster by inactivating the IP3 signaling using genetic tools. We used the "IP3 sponge," composed of the modified ligand-binding domain from the mouse IP3 receptor, which was designed to absorb IP3 in competition with native IP3 receptors. Another tool was a transgene that generates double-stranded RNA against IP3 receptor mRNA. Both inhibitors diminished the sensitivity of flies to trehalose and sucrose, as estimated by behavioral assays and electrophysiological recordings from the sugar receptor cells. The result indicates that IP3 signaling is indispensable for sugar reception in Drosophila.

AB - We evaluated the role of IP3 in sugar taste reception in Drosophila melanogaster by inactivating the IP3 signaling using genetic tools. We used the "IP3 sponge," composed of the modified ligand-binding domain from the mouse IP3 receptor, which was designed to absorb IP3 in competition with native IP3 receptors. Another tool was a transgene that generates double-stranded RNA against IP3 receptor mRNA. Both inhibitors diminished the sensitivity of flies to trehalose and sucrose, as estimated by behavioral assays and electrophysiological recordings from the sugar receptor cells. The result indicates that IP3 signaling is indispensable for sugar reception in Drosophila.

KW - Behavioral responses

KW - Feeding

KW - Gal4 transgene

KW - IP

KW - Inhibitory RNA

KW - Sugar sensation

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