Tau in cerebrospinal fluid: A potential diagnostic marker in Alzheimer's disease

Hiroyuki Arai, Masanori Terajima, Masakazu Miura, Susumu Higuchi, Taro Muramatsu, Nobuo Machida, Hisatomo Seiki, Sadao Takase, Christopher M. Clark, Virginia M.‐Y Lee, John Q. Trojanowski, Hidetada Sasaki

Research output: Contribution to journalArticlepeer-review

279 Citations (Scopus)


Cerebrospinal fluid from 70 patients with Alzheimer's disease (AD) and 96 patients with non‐AD neurological diseases as well as 19 normal control subjects was surveyed by sandwich enzyme‐linked immunosorbent assay to quantitate levels of the microtubule‐associated protein tau in cerebrospinal fluid. The tau level was significantly increased in AD patients as compared with that in patients with non‐AD neurological diseases and control subjects. Increased tau levels were found irrespective of age at onset, apolipoprotein E genotype, and clinical stage. Western blots of AD cerebrospinal fluid proteins revealed two to three tau‐immunoreactive bands with an apparent molecular mass between 50 and 65 kd consistent with phosphorylated cerebrospinal fluid tau. Taken together, our results suggest that cerebrospinal fluid tau might reflect the progressive accumulation of altered tau due to the progressive death of neurons in the AD brain, and that the enzyme‐linked immunosorbent assay of cerebrospinal fluid tau may prove to be a reliable and early diagnostic test for AD.

Original languageEnglish
Pages (from-to)649-652
Number of pages4
JournalAnnals of Neurology
Issue number4
Publication statusPublished - 1995 Oct

ASJC Scopus subject areas

  • Neurology
  • Clinical Neurology


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