Tec protein-tyrosine kinase is involved in the thrombopoietin/c-Mpl signaling pathway

Y. Yamashita, A. Miyazato, R. Shimizu, N. Komatsu, Y. Miura, K. Ozawa, H. Mano

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23 Citations (Scopus)


Thrombopoietin (TPO) is the ligand of the proto-oncogene product, c-Mpl, and supports the growth and maturation of megakaryocytic cells. Although much attention has been paid to the in vivo role of the TPO/c-Mpl system in the context of maintenance of circulating. platelets, still little is understood about how activation of c-Mpl leads to mitogenesis and differentiation inside cells. Tec protein-tyrosine kinase (PTK) is the prototype of a recently emerging subfamily of nonreceptor type PTKs. Tec has been demonstrated to be activated by a wide range of cytokine stimulations. In this paper we show that TPO stimulation also rapidly enhances tyrosine-phosphorylation and activity of the Tee kinase in a human TPO-dependent cell line. In addition, the Vav protein, a blood cell-specific signaling molecule, is shown to be tyrosine-phosphorylated in response to TPO and to be constitutively associated with the Tec protein. From this evidence, we conclude that Tec is involved in the intracellular signaling system of TPO/c-Mpl.

Original languageEnglish
Pages (from-to)211-216
Number of pages6
JournalExperimental Hematology
Issue number3
Publication statusPublished - 1997


  • c-Mpl
  • Tec
  • Thrombopoietin
  • Vav


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