TY - JOUR
T1 - Techno-functional properties and immunomodulatory potential of exopolysaccharide from Lactiplantibacillus plantarum MM89 isolated from human breast milk
AU - Rajoka, Muhammad Shahid Riaz
AU - Mehwish, Hafiza Mahreen
AU - Kitazawa, Haruki
AU - Barba, Francisco J.
AU - Berthelot, Laureline
AU - Umair, Muhammad
AU - Zhu, Qinchang
AU - He, Zhendan
AU - Zhao, Liqing
N1 - Funding Information:
This project was funded by the National Key R&D Program of China (2021YFA0910800 and 2017YFA053900), the National Natural Science Foundation of China (31670360, 81973293 and U1702286), the Science and Technology Program of Guangdong Province (2017B030301016), the Foundation Committee of Basic and Applied Basic Research of Guangdong Province (2019B1515120029), the Shenzhen Science and Technology Project (JCYJ20190808122605563), the Shenzhen Strategic Emerging Industry Development Project funding (ZDSYS201506031617582, KQCX2014052211150878, CXZZ20150529165110750), the Shenzhen Peacock Plan and the SZU Top Ranking Project (86000000210), and the Special Fund for Development of Strategic Emerging Industries in Shenzhen (JCYJ20190808145613154, KQJSCX20180328100801771).
Publisher Copyright:
© 2021
PY - 2022/5/30
Y1 - 2022/5/30
N2 - An exopolysaccharide, designated as MM89-EPS, was isolated from Lactiplantibacillus plantarum MM89. It was comprised of glucose and mannose molecules with an average molecular weight of 138 kDa. FTIR and NMR spectra showed that MM89-EPS had characteristic polysaccharide functional groups. MM89-EPS displayed excellent water solubility and capacities to retain water and oil due to its porous structure. MM89-EPS exhibited no significant cytotoxicity on RAW264.7 cells and showed strong immunomodulatory activity by increasing phagocytosis, acid phosphatase activity, and cytokine production in RAW264.7 cells. Furthermore, an in vivo study revealed that splenic indices, intestinal IgA levels, serum cytokine levels, and lymphocyte proliferation were increased in an MM89-EPS-treated cyclophosphamide-induced immunosuppressed mouse model. To summarize, our results indicate that MM89-EPS can efficiently enhance the immunostimulatory activity of immune cells and an immunosuppressed mouse model. Hence, MM89-EPS may be use as a potential source of immunomodulatory agent in various food products.
AB - An exopolysaccharide, designated as MM89-EPS, was isolated from Lactiplantibacillus plantarum MM89. It was comprised of glucose and mannose molecules with an average molecular weight of 138 kDa. FTIR and NMR spectra showed that MM89-EPS had characteristic polysaccharide functional groups. MM89-EPS displayed excellent water solubility and capacities to retain water and oil due to its porous structure. MM89-EPS exhibited no significant cytotoxicity on RAW264.7 cells and showed strong immunomodulatory activity by increasing phagocytosis, acid phosphatase activity, and cytokine production in RAW264.7 cells. Furthermore, an in vivo study revealed that splenic indices, intestinal IgA levels, serum cytokine levels, and lymphocyte proliferation were increased in an MM89-EPS-treated cyclophosphamide-induced immunosuppressed mouse model. To summarize, our results indicate that MM89-EPS can efficiently enhance the immunostimulatory activity of immune cells and an immunosuppressed mouse model. Hence, MM89-EPS may be use as a potential source of immunomodulatory agent in various food products.
KW - Human breast milk, exopolysaccharide
KW - Immunomodulatory activity
KW - Lactiplantibacillus plantarum
KW - Techno-functional properties
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U2 - 10.1016/j.foodchem.2021.131954
DO - 10.1016/j.foodchem.2021.131954
M3 - Article
C2 - 34973591
AN - SCOPUS:85121907306
SN - 0308-8146
VL - 377
JO - Food Chemistry
JF - Food Chemistry
M1 - 131954
ER -