Telomerase activity and p53-dependent apoptosis in ovarian cancer cells

R. Akeshima; J. Kigawa; M. Takahashi; T. Oishi; Y. Kanamori; H. Itamochi; M. Shimada; S. Kamazawa; S. Sato; N. Terakawa

doi:10.1054/bjoc.2001.1812

Telomerase activity and p53-dependent apoptosis in ovarian cancer cells

R. Akeshima, J. Kigawa, M. Takahashi, T. Oishi, Y. Kanamori, H. Itamochi, M. Shimada, S. Kamazawa, S. Sato, N. Terakawa

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

We conducted the present study to determine the relationship between p53-dependent apoptosis and telomerase activity in ovarian cancer cells. A human ovarian adenocarcinoma cell line, SK-OV-3 that had hornozygous deletion of the p53 gene was used in this study. Wild-type p53 genes were transducted to SK-OV-3 cells with a recombinant adenovirus that contained a wild-type p53 gene (AxCAp53). IC₅₀ to cisplatin (CDDP) was 12.9 μM for SK-OV-3 cells and 9.2 μM for p53 gene-transducted SK-OV-3 cells. The apoptotic index for cells with p53 gene transduction was significantly higher than cells without transduction. Additionally, p53 gene transduction significantly enhanced CDDP-induced apoptosis. Bax protein in SK-OV-3 cells did not differ before and after exposure to CDDP. In SK-OV-3 cells with transduction of the p53 gene, the expression of p53 and Bax proteins increased after exposure to CDDP. Expression of Bcl-xL decreased after exposure to CDDP in SK-OV-3 cells with and without transduction. The telomerase activity in SK-OV-3 cells with the p53 gene was significantly lower compared with the cells without the p53 gene. CDDP exposure did not affect telomerase activity and human telomerase reverse transcriptase (hTERT) expression in both cell lines. We suggest that the p53 gene may relate to telomerase activity, but that p53-dependent apoptosis does not affect the activity.

Original language	English
Pages (from-to)	1551-1555
Number of pages	5
Journal	British Journal of Cancer
Volume	84
Issue number	11
DOIs	https://doi.org/10.1054/bjoc.2001.1812
Publication status	Published - 2001 Jun 1
Externally published	Yes

Keywords

Apoptosis
Ovarian cancer cells
P53
Telomerase

ASJC Scopus subject areas

Oncology
Cancer Research

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1054/bjoc.2001.1812

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@article{ed7cfc8958af4a6383fa54ddb97b10e4,

title = "Telomerase activity and p53-dependent apoptosis in ovarian cancer cells",

abstract = "We conducted the present study to determine the relationship between p53-dependent apoptosis and telomerase activity in ovarian cancer cells. A human ovarian adenocarcinoma cell line, SK-OV-3 that had hornozygous deletion of the p53 gene was used in this study. Wild-type p53 genes were transducted to SK-OV-3 cells with a recombinant adenovirus that contained a wild-type p53 gene (AxCAp53). IC50 to cisplatin (CDDP) was 12.9 μM for SK-OV-3 cells and 9.2 μM for p53 gene-transducted SK-OV-3 cells. The apoptotic index for cells with p53 gene transduction was significantly higher than cells without transduction. Additionally, p53 gene transduction significantly enhanced CDDP-induced apoptosis. Bax protein in SK-OV-3 cells did not differ before and after exposure to CDDP. In SK-OV-3 cells with transduction of the p53 gene, the expression of p53 and Bax proteins increased after exposure to CDDP. Expression of Bcl-xL decreased after exposure to CDDP in SK-OV-3 cells with and without transduction. The telomerase activity in SK-OV-3 cells with the p53 gene was significantly lower compared with the cells without the p53 gene. CDDP exposure did not affect telomerase activity and human telomerase reverse transcriptase (hTERT) expression in both cell lines. We suggest that the p53 gene may relate to telomerase activity, but that p53-dependent apoptosis does not affect the activity.",

keywords = "Apoptosis, Ovarian cancer cells, P53, Telomerase",

author = "R. Akeshima and J. Kigawa and M. Takahashi and T. Oishi and Y. Kanamori and H. Itamochi and M. Shimada and S. Kamazawa and S. Sato and N. Terakawa",

note = "Funding Information: A 150-bp DNA standard was used as an internal telomerase assay standard (ITAS). SiHa cells, originated from cervical squamous cell carcinoma, were used as a positive control. Telomerase activity was quantitated on a Macintosh Quadra 840AV computer, using the public domain National Institutes of Health image program (written by Wayne Rasband at the US National Institutes of Health and available from the Internet by anonymous ftp from zippy.nimh.nih.gov or on floppy disk from NTIS, 5285 Port Royal Rd, Springfield, VA 22161 USA, part number PB93-504648). The intensity of telomerase activity was expressed relative to the above-mentioned internal standard.",

year = "2001",

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doi = "10.1054/bjoc.2001.1812",

language = "English",

volume = "84",

pages = "1551--1555",

journal = "British Journal of Cancer",

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T1 - Telomerase activity and p53-dependent apoptosis in ovarian cancer cells

AU - Akeshima, R.

AU - Kigawa, J.

AU - Takahashi, M.

AU - Oishi, T.

AU - Kanamori, Y.

AU - Itamochi, H.

AU - Shimada, M.

AU - Kamazawa, S.

AU - Sato, S.

AU - Terakawa, N.

N1 - Funding Information: A 150-bp DNA standard was used as an internal telomerase assay standard (ITAS). SiHa cells, originated from cervical squamous cell carcinoma, were used as a positive control. Telomerase activity was quantitated on a Macintosh Quadra 840AV computer, using the public domain National Institutes of Health image program (written by Wayne Rasband at the US National Institutes of Health and available from the Internet by anonymous ftp from zippy.nimh.nih.gov or on floppy disk from NTIS, 5285 Port Royal Rd, Springfield, VA 22161 USA, part number PB93-504648). The intensity of telomerase activity was expressed relative to the above-mentioned internal standard.

PY - 2001/6/1

Y1 - 2001/6/1

N2 - We conducted the present study to determine the relationship between p53-dependent apoptosis and telomerase activity in ovarian cancer cells. A human ovarian adenocarcinoma cell line, SK-OV-3 that had hornozygous deletion of the p53 gene was used in this study. Wild-type p53 genes were transducted to SK-OV-3 cells with a recombinant adenovirus that contained a wild-type p53 gene (AxCAp53). IC50 to cisplatin (CDDP) was 12.9 μM for SK-OV-3 cells and 9.2 μM for p53 gene-transducted SK-OV-3 cells. The apoptotic index for cells with p53 gene transduction was significantly higher than cells without transduction. Additionally, p53 gene transduction significantly enhanced CDDP-induced apoptosis. Bax protein in SK-OV-3 cells did not differ before and after exposure to CDDP. In SK-OV-3 cells with transduction of the p53 gene, the expression of p53 and Bax proteins increased after exposure to CDDP. Expression of Bcl-xL decreased after exposure to CDDP in SK-OV-3 cells with and without transduction. The telomerase activity in SK-OV-3 cells with the p53 gene was significantly lower compared with the cells without the p53 gene. CDDP exposure did not affect telomerase activity and human telomerase reverse transcriptase (hTERT) expression in both cell lines. We suggest that the p53 gene may relate to telomerase activity, but that p53-dependent apoptosis does not affect the activity.

AB - We conducted the present study to determine the relationship between p53-dependent apoptosis and telomerase activity in ovarian cancer cells. A human ovarian adenocarcinoma cell line, SK-OV-3 that had hornozygous deletion of the p53 gene was used in this study. Wild-type p53 genes were transducted to SK-OV-3 cells with a recombinant adenovirus that contained a wild-type p53 gene (AxCAp53). IC50 to cisplatin (CDDP) was 12.9 μM for SK-OV-3 cells and 9.2 μM for p53 gene-transducted SK-OV-3 cells. The apoptotic index for cells with p53 gene transduction was significantly higher than cells without transduction. Additionally, p53 gene transduction significantly enhanced CDDP-induced apoptosis. Bax protein in SK-OV-3 cells did not differ before and after exposure to CDDP. In SK-OV-3 cells with transduction of the p53 gene, the expression of p53 and Bax proteins increased after exposure to CDDP. Expression of Bcl-xL decreased after exposure to CDDP in SK-OV-3 cells with and without transduction. The telomerase activity in SK-OV-3 cells with the p53 gene was significantly lower compared with the cells without the p53 gene. CDDP exposure did not affect telomerase activity and human telomerase reverse transcriptase (hTERT) expression in both cell lines. We suggest that the p53 gene may relate to telomerase activity, but that p53-dependent apoptosis does not affect the activity.

KW - Apoptosis

KW - Ovarian cancer cells

KW - P53

KW - Telomerase

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Telomerase activity and p53-dependent apoptosis in ovarian cancer cells

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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