@article{cfe75e73827b44ac9e6baf4fe49cdfe2,
title = "Ten-m/Odz3 regulates migration and differentiation of chondrogenic ATDC5 cells via RhoA-mediated actin reorganization",
abstract = "Tenascin-like molecule major (Ten-m)/odd Oz (Odz), a type II transmembrane molecule, is well known to modulate neural development. We have reported that Ten-m/Odz3 is expressed in cartilaginous tissues and cells. Actin cytoskeleton and its regulator ras homolog gene family member A (RhoA) are closely associated with chondrogenesis. The present study aimed to evaluate the function and molecular mechanism of Ten-m/Odz3 during chondrogenesis, focusing on RhoA and the actin cytoskeleton. Ten-m/Odz3 was expressed in precartilaginous condensing mesenchyme in mouse limb buds. Ten-m/Odz3 knockdown in ATDC5 induced actin cytoskeleton reorganization and change of cell shape through modulation of RhoA activity and FGF2 expression. Ten-m/Odz3 knockdown suppressed ATDC5 migration and expression of genes associated with chondrogenesis, such as Sox9 and type II collagen, via RhoA. On the other hand, Ten-m/Odz3 knockdown inhibited proliferation of ATDC5 in a RhoA-independent manner. These findings suggest that Ten-m/Odz3 plays an important role in early chondrogenesis regulating RhoA-mediated actin reorganization.",
keywords = "actin cytoskeleton, ATDC5, chondrogenesis, RhoA, Ten-m/Odz3",
author = "Ikuko Takano and Nobuo Takeshita and Michiko Yoshida and Daisuke Seki and Toshihito Oyanagi and Seiji Kimura and Wei Jiang and Kiyo Sasaki and Chisumi Sogi and Masayoshi Kawatsu and Teruko Takano-Yamamoto",
note = "Funding Information: This study was supported by grants from the Japan Society for the Promotion of Science (to Nobuo Takeshita [20791565, 23792411, 26463084, and 18K09828] and to Teruko Takano‐Yamamoto [15H05048]). The authors would like to thank Dr Kenji Hata (Department of Molecular and Cellular Biochemistry, Osaka University Graduate School of Dentistry) for helpful advice. They would also like to thank the Biomedical Research Unit of Tohoku University Hospital for technical equipment support. Funding Information: This study was supported by grants from the Japan Society for the Promotion of Science (to Nobuo Takeshita [20791565, 23792411, 26463084, and 18K09828] and to Teruko Takano-Yamamoto [15H05048]). The authors would like to thank Dr Kenji Hata (Department of Molecular and Cellular Biochemistry, Osaka University Graduate School of Dentistry) for helpful advice. They would also like to thank the Biomedical Research Unit of Tohoku University Hospital for technical equipment support. Publisher Copyright: {\textcopyright} 2020 Wiley Periodicals LLC",
year = "2021",
month = apr,
doi = "10.1002/jcp.30058",
language = "English",
volume = "236",
pages = "2906--2919",
journal = "Journal of Cellular Physiology",
issn = "0021-9541",
publisher = "Wiley-Liss Inc.",
number = "4",
}