TY - JOUR
T1 - Tetrodotoxin does not protect red-spotted newts, Notophthalmus viridescens, from intestinal parasites
AU - Mebs, Dietrich
AU - Yotsu-Yamashita, Mari
AU - Seitz, Hanns Martin
AU - Arakawa, Osamu
N1 - Funding Information:
The help and support of Dr. Andrew L. Mack, Powdermill Nature Reserve, Rector, PA, is highly appreciated. We like to express our sincere thanks to Dr. Kentaro Kawatsu, Osaka Prefectural Institute of Public Health for providing the TTX-antibodies. Christine Elbert has performed excellent immunohistological work. This work was supported in part by the Cabinet Office, Government of Japan, through the Funding Program for the Next Generation of World-Leading Researchers ( LS012 to M. Y. Y.).
PY - 2012/7
Y1 - 2012/7
N2 - Tetrodotoxin (TTX) and its analogue 6-. epiTTX had been detected in the red-spotted newt, . Notophthalmus viridescens. Thirty specimens of a population from Pennsylvania, USA were histologically examined for the presence of intestinal parasites. More than 50% were found to be infected with nematodes (Trichocephalidae), trematodes or cestodes (Pseudophyllidae). The mean values of TTX and 6-. epiTTX in parasitized and in non-parasitized newts were not significantly different. Using a monoclonal antibody-based immunoenzymatic technique, TTX was localized in the intestinal tissue as well as in the parasites indicating that they accumulate the toxin and suggesting that TTX and 6-. epiTTX are not providing protection from parasites to the newts.
AB - Tetrodotoxin (TTX) and its analogue 6-. epiTTX had been detected in the red-spotted newt, . Notophthalmus viridescens. Thirty specimens of a population from Pennsylvania, USA were histologically examined for the presence of intestinal parasites. More than 50% were found to be infected with nematodes (Trichocephalidae), trematodes or cestodes (Pseudophyllidae). The mean values of TTX and 6-. epiTTX in parasitized and in non-parasitized newts were not significantly different. Using a monoclonal antibody-based immunoenzymatic technique, TTX was localized in the intestinal tissue as well as in the parasites indicating that they accumulate the toxin and suggesting that TTX and 6-. epiTTX are not providing protection from parasites to the newts.
KW - 6-Epitetrodotoxin
KW - Intestinal parasites
KW - Newt
KW - Notophthalmus viridescens
KW - Tetrodotoxin
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U2 - 10.1016/j.toxicon.2012.03.017
DO - 10.1016/j.toxicon.2012.03.017
M3 - Article
C2 - 22480787
AN - SCOPUS:84859795650
SN - 0041-0101
VL - 60
SP - 66
EP - 69
JO - Toxicon
JF - Toxicon
IS - 1
ER -