TY - JOUR
T1 - The ADP-ribosylation factor 1 gene is indispensable for mouse embryonic development after implantation
AU - Hayakawa, Natsuki
AU - Ogoh, Honami
AU - Sumiyoshi, Mami
AU - Matsui, Yasuhisa
AU - Nishikawa, Saori
AU - Miyamoto, Kananko
AU - Maede, Yuko
AU - Kiyonari, Hiroshi
AU - Suzuki, Mai
AU - Watanabe, Toshio
N1 - Funding Information:
We thank Dr. Keiko Yasuda, Akira Suzuki, and Satomi Tanaka for technical advice. This work was supported by JSPS KAKENHI Grant Number 24570159 to Toshio Watanabe, a Nara Women’s University Intramural Grant for Project Research to Toshio Watanabe, and a Sasakawa Scientific Research Grant from The Japan Science Society to Honami Ogoh.
Publisher Copyright:
© 2014 Elsevier Inc. All rights reserved.
PY - 2014/10/31
Y1 - 2014/10/31
N2 - ADP-ribosylation factor (Arf) 1 is thought to affect the morphologies of organelles, such as the Golgi apparatus, and regulate protein trafficking pathways. Mice have six Arf isoforms. In knockdown experiments with HeLa cells, no single Arf isoform among Arf1-5 is required for organelle morphologies or any membrane trafficking step. This suggests that the cooperation of two or more Arfs is a general feature. Although many cell biological and biochemical analyses have proven the importance of Arf1, the physiological roles of Arf1 in mice remain unknown. To investigate the activity of Arf1 in vivo, we established Arf1-deficient mice. Arf-/- blastocysts were identified at the expected Mendelian ratio. The appearance of these blastocysts was indistinguishable from that of wild-type and Arf+/- blastocysts, and they grew normally in an in vitro culture system. However, Arf-/- embryos were degenerated at E5.5, and none survived to E12.5, suggesting that they died soon after implantation. These data establish for the first time that the Arf1 gene is indispensable for mouse embryonic development after implantation.
AB - ADP-ribosylation factor (Arf) 1 is thought to affect the morphologies of organelles, such as the Golgi apparatus, and regulate protein trafficking pathways. Mice have six Arf isoforms. In knockdown experiments with HeLa cells, no single Arf isoform among Arf1-5 is required for organelle morphologies or any membrane trafficking step. This suggests that the cooperation of two or more Arfs is a general feature. Although many cell biological and biochemical analyses have proven the importance of Arf1, the physiological roles of Arf1 in mice remain unknown. To investigate the activity of Arf1 in vivo, we established Arf1-deficient mice. Arf-/- blastocysts were identified at the expected Mendelian ratio. The appearance of these blastocysts was indistinguishable from that of wild-type and Arf+/- blastocysts, and they grew normally in an in vitro culture system. However, Arf-/- embryos were degenerated at E5.5, and none survived to E12.5, suggesting that they died soon after implantation. These data establish for the first time that the Arf1 gene is indispensable for mouse embryonic development after implantation.
KW - ADP-ribosylation factor 1 (Arf1)
KW - Developmental defects
KW - Embryogenesis
KW - Gene knockout
KW - Implantation
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U2 - 10.1016/j.bbrc.2014.10.014
DO - 10.1016/j.bbrc.2014.10.014
M3 - Article
C2 - 25305484
AN - SCOPUS:84909994403
SN - 0006-291X
VL - 453
SP - 748
EP - 753
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 4
ER -