@article{b125b0b6dbcb455693c7729c4678dadf,
title = "The advantages of cDNA microarray as an effective tool for identification of reproductive organ-specific genes in a model legume, Lotus japonicus",
abstract = "To understand the molecular mechanisms intrinsic to reproductive organ development a cDNA microarray, fabricated from flower bud cDNA clones, was used to isolate genes, which are specifically expressed during the development of the anther and pistil in Lotus japonicus. Cluster analysis of the microarray data revealed 21 and 111 independent cDNA groups, which were specifically expressed in immature and mature anthers, respectively. RT-PCR was performed to provide a direct assessment of the accuracy and reproducibility of our approach. Confirmation of our results suggests that cDNA microarray technology is an effective tool for identification of novel reproductive organ-specific genes.",
keywords = "Anther-specific gene, cDNA microarray, Lotus japonicus, Pistil-specific gene, Reverse transcriptase polymerase chain reaction",
author = "Makoto Endo and Hitoshi Matsubara and Takao Kokubun and Hiromi Masuko and Yoshihito Takahata and Tohru Tsuchiya and Hiroo Fukuda and Taku Demura and Masao Watanabe",
note = "Funding Information: The authors thank Dr. Tracie K. Matsumoto, Purdue University, for her critical reading of the manuscript. This work was supported in part by a grant from the Ministry of Agriculture, Forestry and Fisheries of Japan (Rice Genome Project MA-2208 and MA-2211) to T.T. and M.W., respectively. The authors are grateful to Dr. Masayoshi Kawaguchi, Niigata University, for providing seeds of L. japonicus B-129 Gifu. We also thank Dr. Hiroyuki Hirano, University of Tokyo, for providing technical advice in analyzing array data. The authors are also grateful to Miss Yuki Nakano and Miss Mizuho Takahashi, Iwate University for technical assistance. We also thank Dr. Toshihiko Hayakawa and Dr. Katsunori Hatakeyama, Tohoku University, for their helpful discussion.",
year = "2002",
month = mar,
day = "13",
doi = "10.1016/S0014-5793(02)02371-2",
language = "English",
volume = "514",
pages = "229--237",
journal = "FEBS Letters",
issn = "0014-5793",
publisher = "Wiley-Blackwell",
number = "2-3",
}