@article{bbcd1fef1ff94d93a01d622d09cdfbf4,
title = "The AVAG1 gene is involved in development of reproductive organs in the ornamental asparagus, Asparagus virgatus",
abstract = "The AGAMOUS (AG)-like gene has been reported to be involved in the formation of the stamens and carpels. The genus Asparagus contains hermaphrodite and dioecious species, and analysis of the AG-like genes in these species may reveal how these different reproductive systems have evolved in this genus. We isolated one AG-like gene, designated AVAG1, from the ornamental hermaphrodite species Asparagus virgatus. Phylogenetic analysis showed that the AVAG1 gene is closely related to HAG1 from Hyacinthus and PeMADS1 from Phalaenopsis. Northern blot analysis showed that AVAG1 transcripts were detected in flower buds, but not in roots, stems or phylloclades. In situ hybridization analyses revealed that the AVAG1 mRNA is expressed specifically in the floral meristem and the developing reproductive organs. Early in flower development, expression of AVAG1 was restricted mainly to the stamens and carpels, with AVAG1 expression in the stamen disappearing at later stages of flower development, although it remained strong in the ovule.",
keywords = "AGAMOUS, Asparagus virgatus, AVAG1, Flower development, MADS box",
author = "Yun, {Pil Yong} and Takuro Ito and Kim, {So Young} and Akira Kanno and Toshiaki Kameya",
note = "Funding Information: Fig. 6A–F Expression of AVAG1 in the developing A. virgatus flower as determined by in situ hybridization. In A–E, DIG-labeled AVAG1 antisense probes were used. The signal is visible throughout the flower meristem (A) and in the stamen and carpel at the early developmental stage (B). The signal was also detected in the Acknowledgements We wish to thank Professor H. Takahashi and Dr. M. Kamada (Tohoku University, Sendai, Japan) for their help and discussions regarding the in situ experiments. We also thank Dr. T. Ochiai (Tohoku University) for helpful discussions and Mr. H. Tokairin for his collaboration in culturing the plants. We are also grateful to Dr. T. Yamaguchi (Fukukaen Nursery & Bulb Co., Mie, Japan), Dr. K. Sudo (National Institute of Floricultural Science, Tsukuba, Japan) and Dr. T. Sonoda (Fukushima Agricultural Experiment Station, Koriyama, Japan) for providing seeds and/or plants of A. virgatus. This work was supported in part by a Grant-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports Science and Technology, Japan.",
year = "2004",
month = may,
doi = "10.1007/s00497-004-0212-1",
language = "English",
volume = "17",
pages = "1--8",
journal = "Sexual Plant Reproduction",
issn = "0934-0882",
publisher = "Springer Verlag",
number = "1",
}