TY - JOUR
T1 - The brain-specific actin-related protein ArpNα interacts with the transcriptional co-repressor CtBP
AU - Oma, Yukako
AU - Nishimori, Katsuhiko
AU - Harata, Masahiko
N1 - Funding Information:
We thank Dr. M. Fujita and Dr. A.E. Wallberg for the generous gift of plasmids. This work was supported by Grants-in-Aid for Science Research from the Ministry for Education, Science, Sports and Culture of Japan and from Kato Memorial Bioscience Foundation. Y.O. thanks the Japan Society for the Promotion of Science (JSPS) for a Young Scientist Fellowship.
PY - 2003/2/7
Y1 - 2003/2/7
N2 - Actin-related protein (Arp) is found in many chromatin remodeling and histone acetyltransferase complexes. We previously identified ArpNα as an isoform of ArpNβ/BAF53, which is included in mammalian SWI/SNF chromatin remodeling complex, and showed that ArpNα is a potential component of the complex. Although it has a structure highly similar to ArpNβ/BAF53, ArpNα is expressed exclusively in brain and in neural differentiated embryonal carcinoma cells. Since ArpNα possesses a region that shows low similarity to ArpNβ/BAF53, we hypothesized that proteins interacting with this region contribute to the ArpNα-specific function in brain. Here we showed that ArpNα, but not ArpNβ/BAF53, interacts with the transcriptional co-repressor CtBP (C-terminal binding protein). Transactivation by the SWI/SNF complex and glucocorticoid receptor was repressed by the CtBP in the presence of ArpNα. These findings suggest that SWI/SNF complex containing ArpNα might regulate certain genes involved in brain development and/or its function differently from SWI/SNF complex containing ArpNβ/BAF53.
AB - Actin-related protein (Arp) is found in many chromatin remodeling and histone acetyltransferase complexes. We previously identified ArpNα as an isoform of ArpNβ/BAF53, which is included in mammalian SWI/SNF chromatin remodeling complex, and showed that ArpNα is a potential component of the complex. Although it has a structure highly similar to ArpNβ/BAF53, ArpNα is expressed exclusively in brain and in neural differentiated embryonal carcinoma cells. Since ArpNα possesses a region that shows low similarity to ArpNβ/BAF53, we hypothesized that proteins interacting with this region contribute to the ArpNα-specific function in brain. Here we showed that ArpNα, but not ArpNβ/BAF53, interacts with the transcriptional co-repressor CtBP (C-terminal binding protein). Transactivation by the SWI/SNF complex and glucocorticoid receptor was repressed by the CtBP in the presence of ArpNα. These findings suggest that SWI/SNF complex containing ArpNα might regulate certain genes involved in brain development and/or its function differently from SWI/SNF complex containing ArpNβ/BAF53.
KW - Actin-related protein
KW - Brain-specific expression
KW - Chromatin
KW - Chromatin remodeling
KW - Co-repressor
KW - CtBP
KW - Protein-protein interaction
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U2 - 10.1016/S0006-291X(02)03073-5
DO - 10.1016/S0006-291X(02)03073-5
M3 - Article
C2 - 12565893
AN - SCOPUS:0037423699
SN - 0006-291X
VL - 301
SP - 521
EP - 528
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -