The C. elegans unc-18 gene encodes a protein expressed in motor neurons

Keiko Gengyo-Ando; Yasuko Kamiya; Ayanori Yamakawa; Ken ichi Kodaira; Kiyoji Nishiwaki; Johji Miwa; Isao Hori; Ryuji Hosono

doi:10.1016/0896-6273(93)90080-B

The C. elegans unc-18 gene encodes a protein expressed in motor neurons

Keiko Gengyo-Ando, Yasuko Kamiya, Ayanori Yamakawa, Ken ichi Kodaira, Kiyoji Nishiwaki, Johji Miwa, Isao Hori, Ryuji Hosono

DEN - Dental Sciences

Research output: Contribution to journal › Article › peer-review

97 Citations (Scopus)

Abstract

The C. elegans unc-18 gene is required to maintain normal acetylcholine levels. We determined the complete structure of an unc-18 cDNA that encodes a protein of 591 highly charged and hydrophilic amino acids. The protein shows sequence similarity with elements of the secretory pathway in the yeast S. cerevisiae. Antibodies raised against a portion of the unc-18-encoded protein (UNC-18) detected a 68 kd soluble antigen on immunoblots and intensely stained all ventral cord motor neurons in situ. These findings suggest that UNC-18 participates in the axonal transport system and influences the acetylcholine flow in motor neurons.

Original language	English
Pages (from-to)	703-711
Number of pages	9
Journal	Neuron
Volume	11
Issue number	4
DOIs	https://doi.org/10.1016/0896-6273(93)90080-B
Publication status	Published - 1993 Oct

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abstract = "The C. elegans unc-18 gene is required to maintain normal acetylcholine levels. We determined the complete structure of an unc-18 cDNA that encodes a protein of 591 highly charged and hydrophilic amino acids. The protein shows sequence similarity with elements of the secretory pathway in the yeast S. cerevisiae. Antibodies raised against a portion of the unc-18-encoded protein (UNC-18) detected a 68 kd soluble antigen on immunoblots and intensely stained all ventral cord motor neurons in situ. These findings suggest that UNC-18 participates in the axonal transport system and influences the acetylcholine flow in motor neurons.",

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AU - Gengyo-Ando, Keiko

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AU - Kodaira, Ken ichi

AU - Nishiwaki, Kiyoji

AU - Miwa, Johji

AU - Hori, Isao

AU - Hosono, Ryuji

PY - 1993/10

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AB - The C. elegans unc-18 gene is required to maintain normal acetylcholine levels. We determined the complete structure of an unc-18 cDNA that encodes a protein of 591 highly charged and hydrophilic amino acids. The protein shows sequence similarity with elements of the secretory pathway in the yeast S. cerevisiae. Antibodies raised against a portion of the unc-18-encoded protein (UNC-18) detected a 68 kd soluble antigen on immunoblots and intensely stained all ventral cord motor neurons in situ. These findings suggest that UNC-18 participates in the axonal transport system and influences the acetylcholine flow in motor neurons.

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The C. elegans unc-18 gene encodes a protein expressed in motor neurons

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