The comprehensive epigenome map of piRNA clusters

Shinpei Kawaoka, Kahori Hara, Keisuke Shoji, Maki Kobayashi, Toru Shimada, Sumio Sugano, Yukihide Tomari, Yutaka Suzuki, Susumu Katsuma

Research output: Contribution to journalArticlepeer-review

26 Citations (Scopus)


PIWI-interacting RNA (piRNA) clusters act as antitransposon/ retrovirus centers. Integration of selfish jumping elements into piRNA clusters generates de novo piRNAs, which in turn exert trans-silencing activity against these elements in animal gonads. To date, neither genome-wide chromatin modification states of piRNA clusters nor a mode for piRNA precursor transcription have been well understood. Here, to understand the chromatin landscape of piRNA clusters and how piRNA precursors are generated, we analyzed the transcriptome, transcription start sites (TSSs) and the chromatin landscape of the BmN4 cell line, which harbors the germ-line piRNA pathway. Notably, our epigenomic map demonstrated the highly euchromatic nature of unique piRNA clusters. RNA polymerase II was enriched for TSSs that transcribe piRNA precursors. piRNA precursors possessed 50-cap structures as well as 30-poly A-tails. Collectively, we envision that the euchromatic, opened nature of unique piRNA clusters or piRNA cluster-associated TSSs allows piRNA clusters to capture new insertions efficiently.

Original languageEnglish
Pages (from-to)1581-1590
Number of pages10
JournalNucleic acids research
Issue number3
Publication statusPublished - 2013 Feb
Externally publishedYes

ASJC Scopus subject areas

  • Genetics


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