The cytoplasmic region of the amyloid β-protein precursor (APP) is necessary and sufficient for the enhanced fast velocity of APP transport by kinesin-1

Maoko Tsukamoto; Kyoko Chiba; Yuriko Sobu; Yuzuha Shiraki; Yuka Okumura; Saori Hata; Akira Kitamura; Tadashi Nakaya; Seiichi Uchida; Masataka Kinjo; Hidenori Taru; Toshiharu Suzuki

doi:10.1002/1873-3468.13204

The cytoplasmic region of the amyloid β-protein precursor (APP) is necessary and sufficient for the enhanced fast velocity of APP transport by kinesin-1

Maoko Tsukamoto, Kyoko Chiba, Yuriko Sobu, Yuzuha Shiraki, Yuka Okumura, Saori Hata, Akira Kitamura, Tadashi Nakaya, Seiichi Uchida, Masataka Kinjo, Hidenori Taru, Toshiharu Suzuki

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Abstract

Amyloid β-protein precursor (APP) is transported mainly by kinesin-1 and at a higher velocity than other kinesin-1 cargos, such as Alcadein α (Alcα); this is denoted by the enhanced fast velocity (EFV). Interaction of the APP cytoplasmic region with kinesin-1, which is essential for EFV transport, is mediated by JNK-interacting protein 1 (JIP1). To determine the roles of interactions between the APP luminal region and cargo components, we monitored transport of chimeric cargo receptors, Alcα (luminal)–APP (cytoplasmic) and APP (luminal)–Alcα (cytoplasmic). Alcα-APP is transported at the EFV, whereas APP-Alcα is transported at the same velocity as wild-type Alcα. Thus, the cytoplasmic region of APP is necessary and sufficient for the EFV of APP transport by kinesin-1.

Original language	English
Pages (from-to)	2716-2724
Number of pages	9
Journal	FEBS Letters
Volume	592
Issue number	16
DOIs	https://doi.org/10.1002/1873-3468.13204
Publication status	Published - 2018 Aug

Keywords

alcadein
APP
axonal transport
calsyntenin
JIP
kinesin-1

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10.1002/1873-3468.13204

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Tsukamoto, M., Chiba, K., Sobu, Y., Shiraki, Y., Okumura, Y., Hata, S., Kitamura, A., Nakaya, T., Uchida, S., Kinjo, M., Taru, H., & Suzuki, T. (2018). The cytoplasmic region of the amyloid β-protein precursor (APP) is necessary and sufficient for the enhanced fast velocity of APP transport by kinesin-1. FEBS Letters, 592(16), 2716-2724. https://doi.org/10.1002/1873-3468.13204

@article{34805c7613934372b8348631595b4b98,

title = "The cytoplasmic region of the amyloid β-protein precursor (APP) is necessary and sufficient for the enhanced fast velocity of APP transport by kinesin-1",

abstract = "Amyloid β-protein precursor (APP) is transported mainly by kinesin-1 and at a higher velocity than other kinesin-1 cargos, such as Alcadein α (Alcα); this is denoted by the enhanced fast velocity (EFV). Interaction of the APP cytoplasmic region with kinesin-1, which is essential for EFV transport, is mediated by JNK-interacting protein 1 (JIP1). To determine the roles of interactions between the APP luminal region and cargo components, we monitored transport of chimeric cargo receptors, Alcα (luminal)–APP (cytoplasmic) and APP (luminal)–Alcα (cytoplasmic). Alcα-APP is transported at the EFV, whereas APP-Alcα is transported at the same velocity as wild-type Alcα. Thus, the cytoplasmic region of APP is necessary and sufficient for the EFV of APP transport by kinesin-1.",

keywords = "alcadein, APP, axonal transport, calsyntenin, JIP, kinesin-1",

author = "Maoko Tsukamoto and Kyoko Chiba and Yuriko Sobu and Yuzuha Shiraki and Yuka Okumura and Saori Hata and Akira Kitamura and Tadashi Nakaya and Seiichi Uchida and Masataka Kinjo and Hidenori Taru and Toshiharu Suzuki",

note = "Funding Information: This work was supported in part by the Strategic Research Program for Brain Sciences from the Japan Agency for Medical Research and Development (JP18 dm017142 h0003) and The Naito Foundation (to SH), by KAKENHI, a Grants-in-Aid for Scientific Research (JP18K07384 to SH, JP18H04851 and JP18H02566 to TS) from JSPS in Japan, and by a Grants-in-Aid for Scientific Research on Innovative Areas-Platform for Advanced Technologies and Research Resources “Advanced Bioimaging Support” to SU. Publisher Copyright: {\textcopyright} 2018 Federation of European Biochemical Societies",

year = "2018",

month = aug,

doi = "10.1002/1873-3468.13204",

language = "English",

volume = "592",

pages = "2716--2724",

journal = "FEBS Letters",

issn = "0014-5793",

publisher = "Wiley-Blackwell",

number = "16",

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Tsukamoto, M, Chiba, K, Sobu, Y, Shiraki, Y, Okumura, Y, Hata, S, Kitamura, A, Nakaya, T, Uchida, S, Kinjo, M, Taru, H & Suzuki, T 2018, 'The cytoplasmic region of the amyloid β-protein precursor (APP) is necessary and sufficient for the enhanced fast velocity of APP transport by kinesin-1', FEBS Letters, vol. 592, no. 16, pp. 2716-2724. https://doi.org/10.1002/1873-3468.13204

TY - JOUR

T1 - The cytoplasmic region of the amyloid β-protein precursor (APP) is necessary and sufficient for the enhanced fast velocity of APP transport by kinesin-1

AU - Tsukamoto, Maoko

AU - Chiba, Kyoko

AU - Sobu, Yuriko

AU - Shiraki, Yuzuha

AU - Okumura, Yuka

AU - Hata, Saori

AU - Kitamura, Akira

AU - Nakaya, Tadashi

AU - Uchida, Seiichi

AU - Kinjo, Masataka

AU - Taru, Hidenori

AU - Suzuki, Toshiharu

N1 - Funding Information: This work was supported in part by the Strategic Research Program for Brain Sciences from the Japan Agency for Medical Research and Development (JP18 dm017142 h0003) and The Naito Foundation (to SH), by KAKENHI, a Grants-in-Aid for Scientific Research (JP18K07384 to SH, JP18H04851 and JP18H02566 to TS) from JSPS in Japan, and by a Grants-in-Aid for Scientific Research on Innovative Areas-Platform for Advanced Technologies and Research Resources “Advanced Bioimaging Support” to SU. Publisher Copyright: © 2018 Federation of European Biochemical Societies

PY - 2018/8

Y1 - 2018/8

N2 - Amyloid β-protein precursor (APP) is transported mainly by kinesin-1 and at a higher velocity than other kinesin-1 cargos, such as Alcadein α (Alcα); this is denoted by the enhanced fast velocity (EFV). Interaction of the APP cytoplasmic region with kinesin-1, which is essential for EFV transport, is mediated by JNK-interacting protein 1 (JIP1). To determine the roles of interactions between the APP luminal region and cargo components, we monitored transport of chimeric cargo receptors, Alcα (luminal)–APP (cytoplasmic) and APP (luminal)–Alcα (cytoplasmic). Alcα-APP is transported at the EFV, whereas APP-Alcα is transported at the same velocity as wild-type Alcα. Thus, the cytoplasmic region of APP is necessary and sufficient for the EFV of APP transport by kinesin-1.

AB - Amyloid β-protein precursor (APP) is transported mainly by kinesin-1 and at a higher velocity than other kinesin-1 cargos, such as Alcadein α (Alcα); this is denoted by the enhanced fast velocity (EFV). Interaction of the APP cytoplasmic region with kinesin-1, which is essential for EFV transport, is mediated by JNK-interacting protein 1 (JIP1). To determine the roles of interactions between the APP luminal region and cargo components, we monitored transport of chimeric cargo receptors, Alcα (luminal)–APP (cytoplasmic) and APP (luminal)–Alcα (cytoplasmic). Alcα-APP is transported at the EFV, whereas APP-Alcα is transported at the same velocity as wild-type Alcα. Thus, the cytoplasmic region of APP is necessary and sufficient for the EFV of APP transport by kinesin-1.

KW - alcadein

KW - APP

KW - axonal transport

KW - calsyntenin

KW - JIP

KW - kinesin-1

UR - http://www.scopus.com/inward/record.url?scp=85052379605&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85052379605&partnerID=8YFLogxK

U2 - 10.1002/1873-3468.13204

DO - 10.1002/1873-3468.13204

M3 - Article

C2 - 30055048

AN - SCOPUS:85052379605

SN - 0014-5793

VL - 592

SP - 2716

EP - 2724

JO - FEBS Letters

JF - FEBS Letters

IS - 16

ER -

The cytoplasmic region of the amyloid β-protein precursor (APP) is necessary and sufficient for the enhanced fast velocity of APP transport by kinesin-1

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Keywords

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