@article{4e6172ac3af84b8cad98084483c28641,
title = "The development of a bioengineered organ germ method",
abstract = "To bioengineer ectodermal organs such as teeth and whisker follicles, we developed a three-dimensional organ-germ culture method. The bioengineered tooth germ generated a structurally correct tooth, after both in vitro organ culture as well as transplantation under a tooth cavity in vivo, showing penetration of blood vessels and nerve fibers. Our method provides a substantial advance in the development of bioengineered organ replacement strategies and regenerative therapies.",
author = "Kazuhisa Nakao and Ritsuko Morita and Yasumitsu Saji and Kentaro Ishida and Yusuke Tomita and Miho Ogawa and Masahiro Saitoh and Yasuhiro Tomooka and Takashi Tsuji",
note = "Funding Information: We thank M. Okabe (Osaka University) for providing the C57BL/6-TgN (act-EGFP) OsbC14-Y01-FM131 mice. We are also grateful to K. Itoh and M. Sugai (Kyoto University) for critical reading of this manuscript. We also thank to T. Katakai (Kyoto University) and Y. Nishi (Nagahama Institute of Bioscience and Technology) for their valuable discussions and encouragement. This work was partially supported by an {\textquoteleft}{\textquoteleft}Academic Frontier{\textquoteright}{\textquoteright} Project for Private Universities to Y.T. and T.T. (2003–2007) and by a Grant-in Aid for Scientific Research in Priority Areas (50339131) to T.T. from MEXT Japan.",
year = "2007",
month = mar,
doi = "10.1038/nmeth1012",
language = "English",
volume = "4",
pages = "227--230",
journal = "Nature Methods",
issn = "1548-7091",
publisher = "Nature Publishing Group",
number = "3",
}