Abstract
Summary: Background: Defining the impact of diabetes and related risk factors on brain cognitive function is critically important for patients with diabetes. Aims: To investigate the alterations in hippocampal serine/threonine kinases signaling in the early phase of type 1 and type 2 diabetic rats. Methods: Early experimental diabetes mellitus was induced in rats with streptozotocin or streptozotocin/high fat. Changes in the phosphorylation of proteins were determined by immunoblotting and immunohistochemistry. Results: Our data showed a pronounced decrease in the phosphorylation of Ca2+/calmodulin-dependent protein kinase II (CaMKII) in the hippocampi of both type 1 and type 2 diabetic rats compared with age-matched control rats. Unexpectedly, we found a significant increase in the phosphorylation of synapsin I (Ser 603) and GluR1 (Ser 831) in the same experiment. In addition, aberrant changes in hippocampal protein kinase C (PKC) and protein kinase A (PKA) signaling in type 1 and type 2 diabetic rats were also found. Moreover, PP1α and PP2A protein levels were decreased in the hippocampus of type 1 diabetic rats, but significantly up-regulated in type 2 diabetic rats. Conclusions: The disturbance of CaMKII/PKA/PKC phosphorylation in the hippocampus is an early change that may be associated with the development and progression of diabetes-related cognitive dysfunction.
| Original language | English |
|---|---|
| Pages (from-to) | 329-336 |
| Number of pages | 8 |
| Journal | CNS Neuroscience and Therapeutics |
| Volume | 19 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - 2013 May |
Keywords
- Brain
- CaMKII
- Diabetes
- Hippocampus
- PKA
- PKC
- Phosphorylation
ASJC Scopus subject areas
- Pharmacology
- Psychiatry and Mental health
- Physiology (medical)
- Pharmacology (medical)
