The essential role of the quasi-long terminal repeat sequence for replication and gene expression of an endogenous pararetrovirus, petunia vein clearing virus

Kazunori Kuriyama, Midori Tabara, Hiromitsu Moriyama, Hideki Takahashi, Toshiyuki Fukuhara

Research output: Contribution to journalArticlepeer-review

Abstract

Petunia vein clearing virus (PVCV) is a type member of the genus Petuvirus within the Caulimoviridae family and is defined as one viral unit consisting of a single open reading frame (ORF) encoding a viral polyprotein and one quasi-long terminal repeat (QTR) sequence. Since some full-length PVCV sequences are found in the petunia genome and a vector for horizontal transmission of PVCV has not been identified yet, PVCV is referred to as an endogenous pararetrovirus. Molecular mechanisms of replication, gene expression and horizontal transmission of endogenous pararetroviruses in plants are elusive. In this study, agroinfiltration experiments using various PVCV infectious clones indicated that the replication (episomal DNA synthesis) and gene expression of PVCV were efficient when the QTR sequences are present on both sides of the ORF. Whereas replacement of the QTR with another promoter and/or terminator is possible for gene expression, it is essential for QTR sequences to be on both sides for viral replication. Although horizontal transmission of PVCV by grafting and biolistic inoculation was previously reported, agroinfiltration is a useful and convenient method for studying its replication and gene expression.

Original languageEnglish
Pages (from-to)405-414
Number of pages10
JournalPlant Biotechnology
Volume39
Issue number4
DOIs
Publication statusPublished - 2022

Keywords

  • QTR
  • agroinfiltration
  • episomal DNA
  • pararetrovirus
  • petunia vein clearing virus

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