The expression of presenilin 1 mRNA in skin fibroblasts and brains from sporadic Alzheimer's disease

Kazuyuki Ikeda; Katsuya Urakami; Hiroyuki Arai; Kenji Wada; Yosuke Wakutani; Yong Ji; Yoshiki Adachi; Akitsugu Okada; Hisanori Kowa; Hidetada Sasaki; Kousaku Ohno; Yuzuru Ohtsuka; Yukihiro Ishikawa; Kenji Nakashima

doi:10.1159/000017246

The expression of presenilin 1 mRNA in skin fibroblasts and brains from sporadic Alzheimer's disease

Kazuyuki Ikeda, Katsuya Urakami, Hiroyuki Arai, Kenji Wada, Yosuke Wakutani, Yong Ji, Yoshiki Adachi, Akitsugu Okada, Hisanori Kowa, Hidetada Sasaki, Kousaku Ohno, Yuzuru Ohtsuka, Yukihiro Ishikawa, Kenji Nakashima

Division of Brain Science

Research output: Contribution to journal › Article › peer-review

11 Citations (Scopus)

Abstract

We examined the expression of presenilin 1 (PS-1) mRNA in cultured skin fibroblasts taken from living patients with Alzheimer's disease (AD) and human brains taken postmortem from AD patients by RT-PCR analysis. The donors of fibroblasts consisted of 28 cases with AD and 19 neurological patient without dementia (CTL). The brains came from 17 cases with AD and 23 cases with CTL. We found that PS-1 mRNA levels in skin fibroblasts of AD patients were significantly higher than those of CTL patients (p < 0.0001). Moreover, we found that PS-1 mRNA levels in human brains with AD were significantly higher than in those with CTL (p < 0.0001). These findings suggest that high levels of PS-1 mRNA in AD may play an important role in developing AD and that the examination of PS-1 mRNA in skin fibroblasts may be helpful for the diagnosis of AD. Copyright (C) 2000 S. Karger AG, Basel.

Original language	English
Pages (from-to)	245-250
Number of pages	6
Journal	Dementia and geriatric cognitive disorders
Volume	11
Issue number	5
DOIs	https://doi.org/10.1159/000017246
Publication status	Published - 2000

Keywords

Human brains
Presenilin 1
Skin fibroblasts
Sporadic Alzheimer's disease
mRNA

ASJC Scopus subject areas

Geriatrics and Gerontology
Cognitive Neuroscience
Psychiatry and Mental health

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1159/000017246

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title = "The expression of presenilin 1 mRNA in skin fibroblasts and brains from sporadic Alzheimer's disease",

abstract = "We examined the expression of presenilin 1 (PS-1) mRNA in cultured skin fibroblasts taken from living patients with Alzheimer's disease (AD) and human brains taken postmortem from AD patients by RT-PCR analysis. The donors of fibroblasts consisted of 28 cases with AD and 19 neurological patient without dementia (CTL). The brains came from 17 cases with AD and 23 cases with CTL. We found that PS-1 mRNA levels in skin fibroblasts of AD patients were significantly higher than those of CTL patients (p < 0.0001). Moreover, we found that PS-1 mRNA levels in human brains with AD were significantly higher than in those with CTL (p < 0.0001). These findings suggest that high levels of PS-1 mRNA in AD may play an important role in developing AD and that the examination of PS-1 mRNA in skin fibroblasts may be helpful for the diagnosis of AD. Copyright (C) 2000 S. Karger AG, Basel.",

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author = "Kazuyuki Ikeda and Katsuya Urakami and Hiroyuki Arai and Kenji Wada and Yosuke Wakutani and Yong Ji and Yoshiki Adachi and Akitsugu Okada and Hisanori Kowa and Hidetada Sasaki and Kousaku Ohno and Yuzuru Ohtsuka and Yukihiro Ishikawa and Kenji Nakashima",

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doi = "10.1159/000017246",

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T1 - The expression of presenilin 1 mRNA in skin fibroblasts and brains from sporadic Alzheimer's disease

AU - Ikeda, Kazuyuki

AU - Urakami, Katsuya

AU - Arai, Hiroyuki

AU - Wada, Kenji

AU - Wakutani, Yosuke

AU - Ji, Yong

AU - Adachi, Yoshiki

AU - Okada, Akitsugu

AU - Kowa, Hisanori

AU - Sasaki, Hidetada

AU - Ohno, Kousaku

AU - Ohtsuka, Yuzuru

AU - Ishikawa, Yukihiro

AU - Nakashima, Kenji

PY - 2000

Y1 - 2000

N2 - We examined the expression of presenilin 1 (PS-1) mRNA in cultured skin fibroblasts taken from living patients with Alzheimer's disease (AD) and human brains taken postmortem from AD patients by RT-PCR analysis. The donors of fibroblasts consisted of 28 cases with AD and 19 neurological patient without dementia (CTL). The brains came from 17 cases with AD and 23 cases with CTL. We found that PS-1 mRNA levels in skin fibroblasts of AD patients were significantly higher than those of CTL patients (p < 0.0001). Moreover, we found that PS-1 mRNA levels in human brains with AD were significantly higher than in those with CTL (p < 0.0001). These findings suggest that high levels of PS-1 mRNA in AD may play an important role in developing AD and that the examination of PS-1 mRNA in skin fibroblasts may be helpful for the diagnosis of AD. Copyright (C) 2000 S. Karger AG, Basel.

AB - We examined the expression of presenilin 1 (PS-1) mRNA in cultured skin fibroblasts taken from living patients with Alzheimer's disease (AD) and human brains taken postmortem from AD patients by RT-PCR analysis. The donors of fibroblasts consisted of 28 cases with AD and 19 neurological patient without dementia (CTL). The brains came from 17 cases with AD and 23 cases with CTL. We found that PS-1 mRNA levels in skin fibroblasts of AD patients were significantly higher than those of CTL patients (p < 0.0001). Moreover, we found that PS-1 mRNA levels in human brains with AD were significantly higher than in those with CTL (p < 0.0001). These findings suggest that high levels of PS-1 mRNA in AD may play an important role in developing AD and that the examination of PS-1 mRNA in skin fibroblasts may be helpful for the diagnosis of AD. Copyright (C) 2000 S. Karger AG, Basel.

KW - Human brains

KW - Presenilin 1

KW - Skin fibroblasts

KW - Sporadic Alzheimer's disease

KW - mRNA

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The expression of presenilin 1 mRNA in skin fibroblasts and brains from sporadic Alzheimer's disease

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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