TY - JOUR
T1 - The function of rhamnose-binding lectin in innate immunity by restricted binding to Gb3
AU - Watanabe, Yasuharu
AU - Tateno, Hiroaki
AU - Nakamura-Tsuruta, Sachiko
AU - Kominami, Junko
AU - Hirabayashi, Jun
AU - Nakamura, Osamu
AU - Watanabe, Tasuku
AU - Kamiya, Hisao
AU - Naganuma, Takako
AU - Ogawa, Tomohisa
AU - Naudé, Ryno J.
AU - Muramoto, Koji
N1 - Funding Information:
We thank Dr Y. Tojo (Shiseido, Tokyo, Japan) for providing us with a gel filtration column PC200S. This work was supported by Grants-in-Aid for Scientific Research (17380122, 20380118) from the Ministry of Education, Science, Sports and Culture of Japan.
PY - 2009/2
Y1 - 2009/2
N2 - l-Rhamnose-binding lectins (RBLs) have been isolated from various kinds of fish and invertebrates and interact with various kinds of bacteria, suggesting RBLs are involved in various inflammatory reactions. We investigated the effect of RBLs from chum salmon (Oncorhynchus keta), named CSL1, 2 and 3, on the peritoneal macrophage cell line from rainbow trout (Oncorhynchus mykiss) (RTM5) and an established fibroblastic-like cell line derived from gonadal tissue of rainbow trout (RTG-2). CSLs were bound to the surface of RTM5 and RTG-2 cells and induced proinflammatory cytokines, including IL-1β1, IL-1β2, TNF-α1, TNF-α2 and IL-8 in both cells by recognizing globotriaosylceramide (Gb3). In addition, CSLs had an opsonic effect on RTM5 cells and this effect was significantly inhibited by l-rhamnose, indicating that CSLs enhanced their phagocytosis by binding to Gb3 on cell surfaces. This is the first finding that Gb3 plays a role in innate immunity by cooperating with natural ligands, RBLs.
AB - l-Rhamnose-binding lectins (RBLs) have been isolated from various kinds of fish and invertebrates and interact with various kinds of bacteria, suggesting RBLs are involved in various inflammatory reactions. We investigated the effect of RBLs from chum salmon (Oncorhynchus keta), named CSL1, 2 and 3, on the peritoneal macrophage cell line from rainbow trout (Oncorhynchus mykiss) (RTM5) and an established fibroblastic-like cell line derived from gonadal tissue of rainbow trout (RTG-2). CSLs were bound to the surface of RTM5 and RTG-2 cells and induced proinflammatory cytokines, including IL-1β1, IL-1β2, TNF-α1, TNF-α2 and IL-8 in both cells by recognizing globotriaosylceramide (Gb3). In addition, CSLs had an opsonic effect on RTM5 cells and this effect was significantly inhibited by l-rhamnose, indicating that CSLs enhanced their phagocytosis by binding to Gb3 on cell surfaces. This is the first finding that Gb3 plays a role in innate immunity by cooperating with natural ligands, RBLs.
KW - Chum salmon
KW - Cytokine
KW - Gb3
KW - Globotriaosylceramide
KW - Innate immunity
KW - Lectin
KW - Opsonic effect
KW - Rhamnose-binding lectin
UR - http://www.scopus.com/inward/record.url?scp=54849437048&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=54849437048&partnerID=8YFLogxK
U2 - 10.1016/j.dci.2008.08.008
DO - 10.1016/j.dci.2008.08.008
M3 - Article
C2 - 18809432
AN - SCOPUS:54849437048
SN - 0145-305X
VL - 33
SP - 187
EP - 197
JO - Developmental and Comparative Immunology
JF - Developmental and Comparative Immunology
IS - 2
ER -