TY - JOUR
T1 - The g.841G>C SNP of FASN gene is associated with fatty acid composition in beef cattle
AU - Hayakawa, Kiri
AU - Sakamoto, Takayuki
AU - Ishii, Atsushi
AU - Yamaji, Keita
AU - Uemoto, Yoshinobu
AU - Sasago, Nanae
AU - Kobayashi, Eiji
AU - Kobayashi, Naohiko
AU - Matsuhashi, Tamako
AU - Maruyama, Shin
AU - Matsumoto, Hirokazu
AU - Oyama, Kenji
AU - Mannen, Hideyuki
AU - Sasazaki, Shinji
N1 - Publisher Copyright:
© 2015 Japanese Society of Animal Science.
PY - 2015/8/1
Y1 - 2015/8/1
N2 - The objective of the current study is to evaluate the association between fatty acid composition and fatty acid synthase gene polymorphisms as responsible mutations. For this purpose, we selected seven previously reported single nucleotide polymorphisms (SNPs) in FASN gene, including one within promoter region (g.841G>C) and six non-synonymous SNPs (g.8805C>T, g.13126C>T, g.15532A>C, g.16024A>G, g.16039C>T, g.17924A>G), and genotyped them in Japanese Black cattle. Genotyping results revealed that g.8805 C>T and g.17924 A>G were monomorphic loci. Genome-wide association analysis including the other five SNPs revealed that only g.841G>C showed significant associations with the percentages of C14:0, C14:1, C16:1 and C18:1 at 5% genome-wide significance level. In order to further evaluate the effect, we genotyped g.841G>C using additional three populations, including two Japanese Black populations and a Holstein cattle population. g.16024A>G was also genotyped and included in the analysis because it has been reported to be associated with fatty acid composition in Japanese Black cattle. In the result of analysis of variance, g.841G>C showed stronger effects on fatty acid percentage than those of g.16024A>G in all populations. These results suggested that g.841G>C would be a responsible mutation for fatty acid composition and contribute to production of high-grade beef as a selection marker in beef cattle.
AB - The objective of the current study is to evaluate the association between fatty acid composition and fatty acid synthase gene polymorphisms as responsible mutations. For this purpose, we selected seven previously reported single nucleotide polymorphisms (SNPs) in FASN gene, including one within promoter region (g.841G>C) and six non-synonymous SNPs (g.8805C>T, g.13126C>T, g.15532A>C, g.16024A>G, g.16039C>T, g.17924A>G), and genotyped them in Japanese Black cattle. Genotyping results revealed that g.8805 C>T and g.17924 A>G were monomorphic loci. Genome-wide association analysis including the other five SNPs revealed that only g.841G>C showed significant associations with the percentages of C14:0, C14:1, C16:1 and C18:1 at 5% genome-wide significance level. In order to further evaluate the effect, we genotyped g.841G>C using additional three populations, including two Japanese Black populations and a Holstein cattle population. g.16024A>G was also genotyped and included in the analysis because it has been reported to be associated with fatty acid composition in Japanese Black cattle. In the result of analysis of variance, g.841G>C showed stronger effects on fatty acid percentage than those of g.16024A>G in all populations. These results suggested that g.841G>C would be a responsible mutation for fatty acid composition and contribute to production of high-grade beef as a selection marker in beef cattle.
KW - FASN
KW - Fatty acid composition
KW - GWAS
KW - Holstein
KW - Japanese Black cattle
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U2 - 10.1111/asj.12357
DO - 10.1111/asj.12357
M3 - Article
C2 - 25597874
AN - SCOPUS:84937074154
SN - 1344-3941
VL - 86
SP - 737
EP - 746
JO - Animal Science Journal
JF - Animal Science Journal
IS - 8
ER -