The gene search system: Its application to functional genomics in Drosophila melanogaster

Toshiro Aigaki, Takashi Ohsako, Gakuta Toba, Ki Hyeon Seong, Takashi Matsuo

Research output: Contribution to journalReview articlepeer-review

13 Citations (Scopus)


In Drosophila melanogaster, gain-of-function mutagenesis utilizing the GAL4-UAS system has been established, allowing identification of genes that may not be easily detectable by loss-of-function screening approaches. The conditional features of misexpression systems are especially useful for studying late-stage biological processes, such as those involving adult behavior or lifespan. The gene search system, incorporating a bidirectional misexpression vector, was used to screen for genes critical for longevity determination. We have identified several genes whose misexpression in adulthood extends the fly's lifespan. Phenotypic characterization of fly lines carrying a misexpression vector, in conjunction with obtaining information about the genomic insertion sites, creates valuable resources for the systematic functional genomics in Drosophila.

Original languageEnglish
Pages (from-to)169-178
Number of pages10
JournalJournal of neurogenetics
Issue number3-4
Publication statusPublished - 2001
Externally publishedYes


  • GAL4-UAS
  • Longevity
  • Misexpression
  • P-element

ASJC Scopus subject areas

  • Genetics
  • Cellular and Molecular Neuroscience


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