The GTPase-deficient Rab27A(Q78L) mutant inhibits melanosome transport in melanocytes through Trapping of Rab27A effector protein Slac2-a/Melanophilin in their cytosol: Development of a novel melanosome-targeting tag

Morié Ishida, Saki P. Arai, Norihiko Ohbayashi, Mitsunori Fukuda

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)

Abstract

Background: A GTPase-deficient Rab27A(Q78L) mutation caused perinuclear melanosome aggregation by an unknown mechanism. Results: Forcible targeting of Rab27A(Q78L) to melanosomes by a novel melanosome-targeting tag restored peripheral melanosome distribution in Rab27A-deficient cells. Conclusion: The GTPase activity of Rab27A is required for its melanosome localization, not for melanosome transport. Significance: These findings provide new insights into the mechanism underlying the spatiotemporal regulation of Rab27A activity.

Original languageEnglish
Pages (from-to)11059-11067
Number of pages9
JournalJournal of Biological Chemistry
Volume289
Issue number16
DOIs
Publication statusPublished - 2014 Apr 18

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