The in vitro effect of leptin on basal and growth hormone-releasing hormone-stimulated growth hormone secretion from the ovine pituitary gland

Sang Gun Roh; Iain J. Clarke; Ru Wei Xu; James W. Goding; Kylie Loneragan; Chen Chen

doi:10.1159/000054385

The in vitro effect of leptin on basal and growth hormone-releasing hormone-stimulated growth hormone secretion from the ovine pituitary gland

Sang Gun Roh, Iain J. Clarke, Ru Wei Xu, James W. Goding, Kylie Loneragan, Chen Chen

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

We have studied the direct effect of leptin on the secretion of GH from the anterior pituitary gland of the sheep. Using primary cultures of ovine pituitary cells, leptin (10^-9-10^-7 M) treatment for 30 min did not affect basal or GHRH (10^-7 M)-stimulated GH secretion. Following treatment for 24 h, a dose of 10^-7 M leptin stimulated basal GH secretion. In contrast, doses of 10^-7 and 10^-8 M leptin inhibited GHRH-stimulated GH secretion after 24 h of treatment. These results suggest that leptin can have long-term effects on the somatotropes, but no acute effect. Furthermore, leptin appears to have opposite effects on basal and GHRH-stimulated GH secretion.

Original language	English
Pages (from-to)	361-364
Number of pages	4
Journal	Neuroendocrinology
Volume	68
Issue number	6
DOIs	https://doi.org/10.1159/000054385
Publication status	Published - 1998 Dec 1
Externally published	Yes

Keywords

Growth hormone
Growth hormone-releasing hormone
Leptin
Ovine
Somatotropes

ASJC Scopus subject areas

Endocrinology, Diabetes and Metabolism
Endocrinology
Endocrine and Autonomic Systems
Cellular and Molecular Neuroscience

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1159/000054385

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title = "The in vitro effect of leptin on basal and growth hormone-releasing hormone-stimulated growth hormone secretion from the ovine pituitary gland",

abstract = "We have studied the direct effect of leptin on the secretion of GH from the anterior pituitary gland of the sheep. Using primary cultures of ovine pituitary cells, leptin (10-9-10-7 M) treatment for 30 min did not affect basal or GHRH (10-7 M)-stimulated GH secretion. Following treatment for 24 h, a dose of 10-7 M leptin stimulated basal GH secretion. In contrast, doses of 10-7 and 10-8 M leptin inhibited GHRH-stimulated GH secretion after 24 h of treatment. These results suggest that leptin can have long-term effects on the somatotropes, but no acute effect. Furthermore, leptin appears to have opposite effects on basal and GHRH-stimulated GH secretion.",

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AU - Roh, Sang Gun

AU - Clarke, Iain J.

AU - Xu, Ru Wei

AU - Goding, James W.

AU - Loneragan, Kylie

AU - Chen, Chen

PY - 1998/12/1

Y1 - 1998/12/1

N2 - We have studied the direct effect of leptin on the secretion of GH from the anterior pituitary gland of the sheep. Using primary cultures of ovine pituitary cells, leptin (10-9-10-7 M) treatment for 30 min did not affect basal or GHRH (10-7 M)-stimulated GH secretion. Following treatment for 24 h, a dose of 10-7 M leptin stimulated basal GH secretion. In contrast, doses of 10-7 and 10-8 M leptin inhibited GHRH-stimulated GH secretion after 24 h of treatment. These results suggest that leptin can have long-term effects on the somatotropes, but no acute effect. Furthermore, leptin appears to have opposite effects on basal and GHRH-stimulated GH secretion.

AB - We have studied the direct effect of leptin on the secretion of GH from the anterior pituitary gland of the sheep. Using primary cultures of ovine pituitary cells, leptin (10-9-10-7 M) treatment for 30 min did not affect basal or GHRH (10-7 M)-stimulated GH secretion. Following treatment for 24 h, a dose of 10-7 M leptin stimulated basal GH secretion. In contrast, doses of 10-7 and 10-8 M leptin inhibited GHRH-stimulated GH secretion after 24 h of treatment. These results suggest that leptin can have long-term effects on the somatotropes, but no acute effect. Furthermore, leptin appears to have opposite effects on basal and GHRH-stimulated GH secretion.

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KW - Somatotropes

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The in vitro effect of leptin on basal and growth hormone-releasing hormone-stimulated growth hormone secretion from the ovine pituitary gland

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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