The influence of anticoagulant and temperature in measurement of plasma hemoglobin concentration for hemolysis test

Xin Yang Li; Itsuro Saito; Shintaro Hara; Takashi Isoyama; Terumi Yurimoto; Yusuke Inoue; Yusuke Abe

doi:10.11239/jsmbe.52.O-544

The influence of anticoagulant and temperature in measurement of plasma hemoglobin concentration for hemolysis test

Xin Yang Li, Itsuro Saito, Shintaro Hara, Takashi Isoyama, Terumi Yurimoto, Yusuke Inoue, Yusuke Abe

Research output: Contribution to journal › Article › peer-review

Abstract

Hemolysis test has been used to evaluate medical device safety and hemocompatibility. However, the influence of measuring plasma hemoglobin concentration could be very complex, making it difficult to get reproducible hemolysis index. The purpose of this study is to compare the variation of plasma hemoglobin concentration by using 3 kinds of anticoagulants (EDTA-2Na, heparin, sodium citrate), and the influence of temperatures(25°C and 37°C). As a means to measure hemoglobin concentration, Cripps method and Porter method were used. The results showed that no significant decrease in hemoglobin concentration was observed in heparin and sodium citrate. However, the hemoglobin concentration in EDTA-2Na decreased. At 37 °C, the decrease in hemoglobin concentration was also observed. Meanwhile, no significant decrease in hemoglobin concentration was observed at 25°C. The reason for the decrease in hemoglobin concentration is considered to be the formation of methemoglobin.

Original language	English
Pages (from-to)	O-544-O-545
Journal	Transactions of Japanese Society for Medical and Biological Engineering
Volume	52
DOIs	https://doi.org/10.11239/jsmbe.52.O-544
Publication status	Published - 2014 Aug 17
Externally published	Yes

Keywords

Anticoagulants
Hemolysis test
Methemoglobin
Temperature

ASJC Scopus subject areas

Biomedical Engineering

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10.11239/jsmbe.52.O-544

Cite this

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title = "The influence of anticoagulant and temperature in measurement of plasma hemoglobin concentration for hemolysis test",

abstract = "Hemolysis test has been used to evaluate medical device safety and hemocompatibility. However, the influence of measuring plasma hemoglobin concentration could be very complex, making it difficult to get reproducible hemolysis index. The purpose of this study is to compare the variation of plasma hemoglobin concentration by using 3 kinds of anticoagulants (EDTA-2Na, heparin, sodium citrate), and the influence of temperatures(25°C and 37°C). As a means to measure hemoglobin concentration, Cripps method and Porter method were used. The results showed that no significant decrease in hemoglobin concentration was observed in heparin and sodium citrate. However, the hemoglobin concentration in EDTA-2Na decreased. At 37 °C, the decrease in hemoglobin concentration was also observed. Meanwhile, no significant decrease in hemoglobin concentration was observed at 25°C. The reason for the decrease in hemoglobin concentration is considered to be the formation of methemoglobin.",

keywords = "Anticoagulants, Hemolysis test, Methemoglobin, Temperature",

author = "Li, {Xin Yang} and Itsuro Saito and Shintaro Hara and Takashi Isoyama and Terumi Yurimoto and Yusuke Inoue and Yusuke Abe",

year = "2014",

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doi = "10.11239/jsmbe.52.O-544",

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T1 - The influence of anticoagulant and temperature in measurement of plasma hemoglobin concentration for hemolysis test

AU - Li, Xin Yang

AU - Saito, Itsuro

AU - Hara, Shintaro

AU - Isoyama, Takashi

AU - Yurimoto, Terumi

AU - Inoue, Yusuke

AU - Abe, Yusuke

PY - 2014/8/17

Y1 - 2014/8/17

N2 - Hemolysis test has been used to evaluate medical device safety and hemocompatibility. However, the influence of measuring plasma hemoglobin concentration could be very complex, making it difficult to get reproducible hemolysis index. The purpose of this study is to compare the variation of plasma hemoglobin concentration by using 3 kinds of anticoagulants (EDTA-2Na, heparin, sodium citrate), and the influence of temperatures(25°C and 37°C). As a means to measure hemoglobin concentration, Cripps method and Porter method were used. The results showed that no significant decrease in hemoglobin concentration was observed in heparin and sodium citrate. However, the hemoglobin concentration in EDTA-2Na decreased. At 37 °C, the decrease in hemoglobin concentration was also observed. Meanwhile, no significant decrease in hemoglobin concentration was observed at 25°C. The reason for the decrease in hemoglobin concentration is considered to be the formation of methemoglobin.

AB - Hemolysis test has been used to evaluate medical device safety and hemocompatibility. However, the influence of measuring plasma hemoglobin concentration could be very complex, making it difficult to get reproducible hemolysis index. The purpose of this study is to compare the variation of plasma hemoglobin concentration by using 3 kinds of anticoagulants (EDTA-2Na, heparin, sodium citrate), and the influence of temperatures(25°C and 37°C). As a means to measure hemoglobin concentration, Cripps method and Porter method were used. The results showed that no significant decrease in hemoglobin concentration was observed in heparin and sodium citrate. However, the hemoglobin concentration in EDTA-2Na decreased. At 37 °C, the decrease in hemoglobin concentration was also observed. Meanwhile, no significant decrease in hemoglobin concentration was observed at 25°C. The reason for the decrease in hemoglobin concentration is considered to be the formation of methemoglobin.

KW - Anticoagulants

KW - Hemolysis test

KW - Methemoglobin

KW - Temperature

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ER -

The influence of anticoagulant and temperature in measurement of plasma hemoglobin concentration for hemolysis test

Abstract

Keywords

ASJC Scopus subject areas

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