The influence of mesenchymal stem cell growth medium for isolation of STRO-1 positive cells from human periodontal ligament

Naoki Endo; Yoko Kobayashi; Daisuke Nishihara; Masaaki Iwamatsu; Masashi Komatsu; Masahiko Kikuchi

doi:10.1007/978-4-431-54070-0_28

The influence of mesenchymal stem cell growth medium for isolation of STRO-1 positive cells from human periodontal ligament

Naoki Endo, Yoko Kobayashi, Daisuke Nishihara, Masaaki Iwamatsu, Masashi Komatsu, Masahiko Kikuchi

Research output: Chapter in Book/Report/Conference proceeding › Chapter

Abstract

In this study, we examined the influence of mesenchymal stem cells growth medium (MSCGM) for STRO-1 positive cells derived from periodontal ligament (PDL) compared with DMEM. PDL cells cultured in DMEM showed spindle shape. On the other hand, PDL cells cultured in MSCGM for 7 days showed colony formation. The isolation rate of STRO-1 positive cells in MSCGM was 1.57 times higher than that in DMEM in 7 days culture and 2.49 times higher in 14 days culture. It is suggested that MSCGM is effective to increase the isolation rate of STRO-1 positive cells from PDL.

Original language	English
Title of host publication	Interface Oral Health Science 2011
Publisher	Springer Japan
Pages	122-124
Number of pages	3
ISBN (Electronic)	9784431540700
ISBN (Print)	9784431540694
DOIs	https://doi.org/10.1007/978-4-431-54070-0_28
Publication status	Published - 2012 Jan 1

Keywords

Immunocytochemistry
Isolation rate
Mesenchymal stem cells
Periodontal ligament
STRO-1

ASJC Scopus subject areas

Dentistry(all)

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10.1007/978-4-431-54070-0_28

Cite this

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title = "The influence of mesenchymal stem cell growth medium for isolation of STRO-1 positive cells from human periodontal ligament",

abstract = "In this study, we examined the influence of mesenchymal stem cells growth medium (MSCGM) for STRO-1 positive cells derived from periodontal ligament (PDL) compared with DMEM. PDL cells cultured in DMEM showed spindle shape. On the other hand, PDL cells cultured in MSCGM for 7 days showed colony formation. The isolation rate of STRO-1 positive cells in MSCGM was 1.57 times higher than that in DMEM in 7 days culture and 2.49 times higher in 14 days culture. It is suggested that MSCGM is effective to increase the isolation rate of STRO-1 positive cells from PDL. ",

keywords = "Immunocytochemistry, Isolation rate, Mesenchymal stem cells, Periodontal ligament, STRO-1",

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AU - Endo, Naoki

AU - Kobayashi, Yoko

AU - Nishihara, Daisuke

AU - Iwamatsu, Masaaki

AU - Komatsu, Masashi

AU - Kikuchi, Masahiko

PY - 2012/1/1

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N2 - In this study, we examined the influence of mesenchymal stem cells growth medium (MSCGM) for STRO-1 positive cells derived from periodontal ligament (PDL) compared with DMEM. PDL cells cultured in DMEM showed spindle shape. On the other hand, PDL cells cultured in MSCGM for 7 days showed colony formation. The isolation rate of STRO-1 positive cells in MSCGM was 1.57 times higher than that in DMEM in 7 days culture and 2.49 times higher in 14 days culture. It is suggested that MSCGM is effective to increase the isolation rate of STRO-1 positive cells from PDL.

AB - In this study, we examined the influence of mesenchymal stem cells growth medium (MSCGM) for STRO-1 positive cells derived from periodontal ligament (PDL) compared with DMEM. PDL cells cultured in DMEM showed spindle shape. On the other hand, PDL cells cultured in MSCGM for 7 days showed colony formation. The isolation rate of STRO-1 positive cells in MSCGM was 1.57 times higher than that in DMEM in 7 days culture and 2.49 times higher in 14 days culture. It is suggested that MSCGM is effective to increase the isolation rate of STRO-1 positive cells from PDL.

KW - Immunocytochemistry

KW - Isolation rate

KW - Mesenchymal stem cells

KW - Periodontal ligament

KW - STRO-1

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The influence of mesenchymal stem cell growth medium for isolation of STRO-1 positive cells from human periodontal ligament

Abstract

Keywords

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