The INO80 complex is required for damage-induced recombination

Satoshi Kawashima; Hideaki Ogiwara; Shusuke Tada; Masahiko Harata; Ulrike Wintersberger; Takemi Enomoto; Masayuki Seki

doi:10.1016/j.bbrc.2007.02.036

The INO80 complex is required for damage-induced recombination

Satoshi Kawashima, Hideaki Ogiwara, Shusuke Tada, Masahiko Harata, Ulrike Wintersberger, Takemi Enomoto, Masayuki Seki

AGR - Agricultural Chemistry

Research output: Contribution to journal › Article › peer-review

31 Citations (Scopus)

Abstract

The budding yeast INO80 complex has a role in remodeling chromatin structure, and the SWR1 complex replaces a H2A/H2B dimer with a variant dimer, H2A.Z (Htz1)/H2B. It has been reported that these chromatin remodeling complexes contain Arp4 (actin-related protein) and actin in common and are recruited to HO endonuclease-induced DNA double-strand break (DSB) site. Reportedly, Ino80 can evict nucleosomes surrounding a HO-induced DSB; however, it has no apparent role to play in the repair of HO-induced DSB. Here we show that an essential factor for INO80 chromatin remodeling activity, Arp8, is involved in damage-induced sister chromatid recombination and interchromosomal recombination between heteroalleles. In contrast, arp6 mutants are proficient for recombination, indicating that the SWR1 complex does not promote recombination. Our data suggest that the remodeling of chromatin by the INO80 complex facilitates efficient homologus recombination upon DNA damages.

Original language	English
Pages (from-to)	835-841
Number of pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	355
Issue number	3
DOIs	https://doi.org/10.1016/j.bbrc.2007.02.036
Publication status	Published - 2007 Apr 13

Keywords

Arp4
Arp5
Arp6
Arp8
Chromatin remodeling
INO80
NuA4
Recombination
Repair
SWR1

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10.1016/j.bbrc.2007.02.036

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title = "The INO80 complex is required for damage-induced recombination",

abstract = "The budding yeast INO80 complex has a role in remodeling chromatin structure, and the SWR1 complex replaces a H2A/H2B dimer with a variant dimer, H2A.Z (Htz1)/H2B. It has been reported that these chromatin remodeling complexes contain Arp4 (actin-related protein) and actin in common and are recruited to HO endonuclease-induced DNA double-strand break (DSB) site. Reportedly, Ino80 can evict nucleosomes surrounding a HO-induced DSB; however, it has no apparent role to play in the repair of HO-induced DSB. Here we show that an essential factor for INO80 chromatin remodeling activity, Arp8, is involved in damage-induced sister chromatid recombination and interchromosomal recombination between heteroalleles. In contrast, arp6 mutants are proficient for recombination, indicating that the SWR1 complex does not promote recombination. Our data suggest that the remodeling of chromatin by the INO80 complex facilitates efficient homologus recombination upon DNA damages.",

keywords = "Arp4, Arp5, Arp6, Arp8, Chromatin remodeling, INO80, NuA4, Recombination, Repair, SWR1",

author = "Satoshi Kawashima and Hideaki Ogiwara and Shusuke Tada and Masahiko Harata and Ulrike Wintersberger and Takemi Enomoto and Masayuki Seki",

note = "Funding Information: This work was supported by Grants-in-Aid for Scientific Research on Priority Areas from the Ministry of Education, Culture, Sports, Science and Technology of Japan. ",

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AU - Kawashima, Satoshi

AU - Ogiwara, Hideaki

AU - Tada, Shusuke

AU - Harata, Masahiko

AU - Wintersberger, Ulrike

AU - Enomoto, Takemi

AU - Seki, Masayuki

N1 - Funding Information: This work was supported by Grants-in-Aid for Scientific Research on Priority Areas from the Ministry of Education, Culture, Sports, Science and Technology of Japan.

PY - 2007/4/13

Y1 - 2007/4/13

N2 - The budding yeast INO80 complex has a role in remodeling chromatin structure, and the SWR1 complex replaces a H2A/H2B dimer with a variant dimer, H2A.Z (Htz1)/H2B. It has been reported that these chromatin remodeling complexes contain Arp4 (actin-related protein) and actin in common and are recruited to HO endonuclease-induced DNA double-strand break (DSB) site. Reportedly, Ino80 can evict nucleosomes surrounding a HO-induced DSB; however, it has no apparent role to play in the repair of HO-induced DSB. Here we show that an essential factor for INO80 chromatin remodeling activity, Arp8, is involved in damage-induced sister chromatid recombination and interchromosomal recombination between heteroalleles. In contrast, arp6 mutants are proficient for recombination, indicating that the SWR1 complex does not promote recombination. Our data suggest that the remodeling of chromatin by the INO80 complex facilitates efficient homologus recombination upon DNA damages.

AB - The budding yeast INO80 complex has a role in remodeling chromatin structure, and the SWR1 complex replaces a H2A/H2B dimer with a variant dimer, H2A.Z (Htz1)/H2B. It has been reported that these chromatin remodeling complexes contain Arp4 (actin-related protein) and actin in common and are recruited to HO endonuclease-induced DNA double-strand break (DSB) site. Reportedly, Ino80 can evict nucleosomes surrounding a HO-induced DSB; however, it has no apparent role to play in the repair of HO-induced DSB. Here we show that an essential factor for INO80 chromatin remodeling activity, Arp8, is involved in damage-induced sister chromatid recombination and interchromosomal recombination between heteroalleles. In contrast, arp6 mutants are proficient for recombination, indicating that the SWR1 complex does not promote recombination. Our data suggest that the remodeling of chromatin by the INO80 complex facilitates efficient homologus recombination upon DNA damages.

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KW - Arp5

KW - Arp6

KW - Arp8

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KW - NuA4

KW - Recombination

KW - Repair

KW - SWR1

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JO - Biochemical and Biophysical Research Communications

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ER -

The INO80 complex is required for damage-induced recombination

Abstract

Keywords

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